Analytical and Bioanalytical Chemistry

, Volume 406, Issue 25, pp 6373–6383 | Cite as

Analysis of N-acylhomoserine lactone dynamics in continuous cultures of Pseudomonas putida IsoF by use of ELISA and UHPLC/qTOF-MS-derived measurements and mathematical models

  • Katharina Buddrus-Schiemann
  • Martin Rieger
  • Marlene Mühlbauer
  • Maria Vittoria Barbarossa
  • Christina Kuttler
  • Burkhard A. Hense
  • Michael Rothballer
  • Jenny Uhl
  • Juliano R. Fonseca
  • Philippe Schmitt-Kopplin
  • Michael Schmid
  • Anton Hartmann
Research Paper

Abstract

In this interdisciplinary approach, the dynamics of production and degradation of the quorum sensing signal 3-oxo-decanoylhomoserine lactone were studied for continuous cultures of Pseudomonas putida IsoF. The signal concentrations were quantified over time by use of monoclonal antibodies and ELISA. The results were verified by use of ultra-high-performance liquid chromatography. By use of a mathematical model we derived quantitative values for non-induced and induced signal production rate per cell. It is worthy of note that we found rather constant values for different rates of dilution in the chemostat, and the values seemed close to those reported for batch cultures. Thus, the quorum-sensing system in P. putida IsoF is remarkably stable under different environmental conditions. In all chemostat experiments, the signal concentration decreased strongly after a peak, because emerging lactonase activity led to a lower concentration under steady-state conditions. This lactonase activity probably is quorum sensing-regulated. The potential ecological implication of such unique regulation is discussed.

Keywords

Pseudomonas putida IsoF Continuous culture N-acylhomoserine lactones Mathematical modelling ELISA Quorum sensing 

Supplementary material

216_2014_8063_MOESM1_ESM.pdf (54 kb)
ESM 1(PDF 53 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Katharina Buddrus-Schiemann
    • 1
  • Martin Rieger
    • 1
  • Marlene Mühlbauer
    • 1
  • Maria Vittoria Barbarossa
    • 2
  • Christina Kuttler
    • 3
  • Burkhard A. Hense
    • 4
  • Michael Rothballer
    • 1
  • Jenny Uhl
    • 5
  • Juliano R. Fonseca
    • 5
  • Philippe Schmitt-Kopplin
    • 5
    • 6
  • Michael Schmid
    • 1
  • Anton Hartmann
    • 1
  1. 1.Research Unit Microbe-Plant Interactions, Helmholtz Zentrum MünchenGerman Research Centre for Environmental Health (GmbH)NeuherbergGermany
  2. 2.Bolyai InstituteUniversity of SzegedSzegedHungary
  3. 3.Centre for Mathematical SciencesTechnische Universität MünchenGarchingGermany
  4. 4.Institute of Computational Biology, Helmholtz Zentrum MünchenGerman Research Centre for Environmental Health (GmbH)NeuherbergGermany
  5. 5.Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum MünchenGerman Research Centre for Environmental Health (GmbH)NeuherbergGermany
  6. 6.Analytical Food ChemistryTechnische Universität MünchenFreising-WeihenstephanGermany

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