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Validation of an LC-MS/MS method for the quantitative determination of a novel fungicide: pyraoxystrobin in rat plasma and its application to a toxicokinetics study

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Abstract

A simple, sensitive, and reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed for determination of pyraoxystrobin in rat plasma and applied to a toxicokinetics study. The separation was performed by gradient elution on a Luna 5 μ C18 (2) 100 Å column (50 × 4.6 mm I.D., 5 μm) with mobile phase: water (0.1 % formic acid, v/v)/acetonitrile (0.1 % formic acid, v/v), followed by quantification with a mass detector in multiple reaction monitoring (MRM) mode using ESI as an interface. The calibration curve was linear over a concentration range of 1.00–200 ng/mL. The recovery for pyraoxystrobin ranged from 101.4 to 108.2 %. The intraday bias and precision ranged from −9.3 to 8.1 % and from 0.7 to 8.4 %, respectively, and the interday bias and precision ranged from −0.3 to 4.0 % and from 4.4 to 7.2 %, respectively. The toxicokinetics of pyraoxystrobin after single 100 and 1,000 mg/kg oral doses were studied in rats.

The chromatogram of pyraoxystrobin highest calibration standard (ULOQ) extract.

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Authors and Affiliations

  1. Department of Analytical Chemistry, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China

    Lihong Lin & Xingjie Guo

  2. Supervision and Test Center for Pesticide Safety Evaluation and Quality Control, 600 Shenliao Road, Shenyang, 110141, China

    Lihong Lin, Wei Yu, Yanan Pang, Changsong Sun, Qinghe Meng & Xiaolei Li

Authors
  1. Lihong Lin
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  2. Wei Yu
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  3. Yanan Pang
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  4. Changsong Sun
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  5. Qinghe Meng
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  6. Xiaolei Li
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  7. Xingjie Guo
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Correspondence to Lihong Lin or Xingjie Guo.

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Lin, L., Yu, W., Pang, Y. et al. Validation of an LC-MS/MS method for the quantitative determination of a novel fungicide: pyraoxystrobin in rat plasma and its application to a toxicokinetics study. Anal Bioanal Chem 406, 5521–5526 (2014). https://doi.org/10.1007/s00216-014-7976-4

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  • DOI: https://doi.org/10.1007/s00216-014-7976-4

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