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Analytical and Bioanalytical Chemistry

, Volume 406, Issue 22, pp 5339–5346 | Cite as

Quantification of genetically modified soya using strong anion exchange chromatography and time-of-flight mass spectrometry

  • Po-Chih Chang
  • P. Muralidhar Reddy
  • Yen-Peng HoEmail author
Research Paper

Abstract

Stable-isotope dimethyl labeling was applied to the quantification of genetically modified (GM) soya. The herbicide-resistant gene-related protein 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) was labeled using a dimethyl labeling reagent, formaldehyde-H2 or -D2. The identification and quantification of CP4 EPSPS was performed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The CP4 EPSPS protein was separated from high abundance proteins using strong anion exchange chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Then, the tryptic peptides from the samples and reference were labeled with formaldehyde-H2 and formaldehyde-D2, respectively. The two labeled pools were mixed and analyzed using MALDI-MS. The data showed a good correlation between the peak ratio of the H- and D-labeled peptides and the GM soya percentages at 0.5, 1, 3, and 5 %, with R 2 of 0.99. The labeling reagents are readily available. The labeling experiments and the detection procedures are simple. The approach is useful for the quantification of GM soya at a level as low as 0.5 %.

Keywords

Genetically modified soya Dimethyl labeling reagent Matrix-assisted laser desorption/ionization mass spectrometry Quantification Anion exchange chromatography 

Notes

Acknowledgments

The authors thank the Ministry of Science and Technology of the Republic of China for supporting this research financially.

Supplementary material

216_2014_7965_MOESM1_ESM.pdf (36 kb)
ESM 1 (PDF 36 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Po-Chih Chang
    • 1
  • P. Muralidhar Reddy
    • 2
  • Yen-Peng Ho
    • 1
    Email author
  1. 1.Department of ChemistryNational Dong Hwa UniversityHualienRepublic of China
  2. 2.Department of Chemistry, Nizam CollegeOsmania UniversityHyderabadIndia

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