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Analytical and Bioanalytical Chemistry

, Volume 406, Issue 22, pp 5283–5292 | Cite as

Validation of an assay for the determination of levoglucosan and associated monosaccharide anhydrides for the quantification of wood smoke in atmospheric aerosol

  • Rebecca L. Cordell
  • Iain R. White
  • Paul S. MonksEmail author
Research Paper

Abstract

Biomass burning is becoming an increasing contributor to atmospheric particulate matter, and concern is increasing over the detrimental health effects of inhaling such particles. Levoglucosan and related monosaccharide anhydrides (MAs) can be used as tracers of the contribution of wood burning to total particulate matter. An improved gas chromatography–mass spectrometry method to quantify atmospheric levels of MAs has been developed and, for the first-time, fully validated. The method uses an optimised, low-volume methanol extraction, derivitisation by trimethylsilylation and analysis with high-throughput gas chromatography–mass spectrometry (GC–MS). Recovery of approximately 90 % for levoglucosan, and 70 % for the isomers galactosan and mannosan, was achieved using spiked blank filters estimates. The method was extensively validated to ensure that the precision of the method over five experimental replicates on five repeat experimental occasions was within 15 % for low, mid and high concentrations and accuracy between 85 and 115 %. The lower limit of quantification (LLOQ) was 0.21 and 1.05 ng m−3 for levoglucosan and galactosan/mannosan, respectively, where the assay satisfied precisions of ≤20 % and accuracies 80–120 %. The limit of detection (LOD) for all analytes was 0.105 ng m−3. The stability of the MAs, once deposited on aerosol filters, was high over the short term (4 weeks) at room temperature and over longer periods (3 months) when stored at −20 °C. The method was applied to determine atmospheric levels of MAs at an urban background site in Leicester (UK) for a month. Mean concentrations of levoglucosan over the month of May were 21.4 ± 18.3 ng m−3, 7.5 ± 6.1 ng m−3 mannosan and 1.8 ± 1.3 ng m−3 galactosan.

Figure

Monosaccharide anhydride levels and percent contribution to PM10 from filter punches taken every 24 h at an urban background site in Leicester, analysed by GC–MS. G galactosan, M mannosan, L levoglucosan

Keywords

Levoglucosan Monosaccharide anhydrides GC–MS Wood burning 

Notes

Acknowledgments

This study was funded under the Joaquin (Joint Air Quality Initiative), an EU cooperation project supported by the INTERREG IVB North West Europe programme (www.nweurope.eu). We are grateful to the anonymous referees for their detailed and constructive comments.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Rebecca L. Cordell
    • 1
  • Iain R. White
    • 1
  • Paul S. Monks
    • 1
    Email author
  1. 1.Department of ChemistryUniversity of LeicesterLeicesterUK

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