Effects of an iron-based fuel-borne catalyst and a diesel particle filter on exhaust toxicity in lung cells in vitro
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Metal-containing fuel additives catalyzing soot combustion in diesel particle filters are used in a widespread manner, and with the growing popularity of diesel vehicles, their application is expected to increase in the near future. Detailed investigation into how such additives affect exhaust toxicity is therefore necessary and has to be performed before epidemiological evidence points towards adverse effects of their application. The present study investigates how the addition of an iron-based fuel additive (Satacen®3, 40 ppm Fe) to low-sulfur diesel affects the in vitro cytotoxic, oxidative, (pro-)inflammatory, and mutagenic activity of the exhaust of a passenger car operated under constant, low-load conditions by exposing a three-dimensional model of the human airway epithelium to complete exhaust at the air–liquid interface. We could show that the use of the iron catalyst without and with filter technology has positive as well as negative effects on exhaust toxicity compared to exhaust with no additives: it decreases the oxidative and, compared to a non-catalyzed diesel particle filter, the mutagenic potential of diesel exhaust, but increases (pro-)inflammatory effects. The presence of a diesel particle filter also influences the impact of Satacen®3 on exhaust toxicity, and the proper choice of the filter type to be used is of importance with regards to exhaust toxicity.
KeywordsExhaust exposures Iron catalyst Diesel particle filter 3D lung cell model Air–liquid interface
The authors would like to acknowledge the financial support of the Swiss Federal Office for the Environment, Erdölvereinigung EV and VSS lubes, as well as the Adolphe Merkle Foundation, and furthermore, the Bern University of Applied Sciences, the Institute of Aerosol and Sensor Technology, Northwestern Switzerland, and the University of Rouen for technical assistance. We would also like to thank Dr. Gruenert (University of California, San Francisco) for providing the 16HBE14o- cell line.
Conflict of interest
Andreas Mayer is the owner and general manager of “TTM Andreas Mayer,” Switzerland, an emission consulting company. As all the other authors, however, he declares to have no conflicts of interest.
- 3.Ghio AJ, Sobus JR, Pleil JD, Madden MC (2012) Controlled human exposures to diesel exhaust. Swiss Med Wkly 142. doi: 10.4414/smw.2012.13597
- 7.Mayer A, Czerwinski J, Wichser A, Ulrich A, Kasper M, Mooney J (2010) Metal-oxide particles in combustion engine exhaust. SAE Technical Papers 2010-01-0792Google Scholar
- 15.Heeb NV, Schmid P, Kohler M, Gujer E, Zennegg M, Wenger D, Wichser A, Ulrich A, Gfeller U, Honegger P, Zeyer K, Emmenegger L, Petermann JL, Czerwinski J, Mosimann T, Kasper M, Mayer A (2008) Secondary effects of catalytic diesel particulate filters: conversion of PAHs versus formation of Nitro-PAHs. Environ Sci Technol 42(10):3773–3779. doi: 10.1021/Es7026949 CrossRefGoogle Scholar
- 16.Heeb NV, Schmid P, Kohler M, Gujer E, Zennegg M, Wenger D, Wichser A, Ulrich A, Gfeller U, Honegger P, Zeyer K, Emmenegger L, Petermann JL, Czerwinski J, Mosimann T, Kasper M, Mayer A (2010) Impact of low- and high-oxidation diesel particulate filters on genotoxic exhaust constituents. Environ Sci Technol 44(3):1078–1084. doi: 10.1021/Es9019222 CrossRefGoogle Scholar
- 17.Heeb NV, Zennegg M, Haag R, Wichser A, Schmid P, Seiler C, Ulrich A, Honegger P, Zeyer K, Emmenegger L, Bonsack P, Zimmerli Y, Czerwinski J, Kasper M, Mayer A (2013) PCDD/F formation in an iron/potassium-catalyzed diesel particle filter. Environ Sci Technol 47(12):6510–6517. doi: 10.1021/es400760h Google Scholar
- 23.Morin JP, Hasson V, Fall M, Papaioanou E, Preterre D, Gouriou F, Keravec V, Konstandopoulos A, Dionnet F (2008) Prevalidation of in vitro continuous flow exposure systems as alternatives to in vivo inhalation safety evaluation experimentations: outcome from MAAPHRI-PCRD5 research program. Exp Toxicol Pathol 60(2–3):195–205. doi: 10.1016/j.etp.2008.01.007 CrossRefGoogle Scholar
- 24.Muller L, Comte P, Czerwinski J, Kasper M, Mayer ACR, Gehr P, Burtscher H, Morin JP, Konstandopoulos A, Rothen-Rutishauser B (2010) New exposure system to evaluate the toxicity of (Scooter) exhaust emissions in lung cells in vitro. Environ Sci Technol 44(7):2632–2638. doi: 10.1021/Es903146g CrossRefGoogle Scholar
- 27.Steiner S, Mueller L, Popovicheva OB, Raemy DO, Czerwinski J, Comte P, Mayer A, Gehr P, Rothen-Rutishauser B, Clift MJD (2012) Cerium dioxide nanoparticles can interfere with the associated cellular mechanistic response to diesel exhaust exposure. Toxicol Lett 214(2):218–225. doi: 10.1016/j.toxlet.2012.08.026 CrossRefGoogle Scholar
- 28.Muller L, Comte P, Czerwinski J, Kasper M, Mayer ACR, Schmid A, Rosinus L, Clift MJD, Steiner S, Gehr P, Rothen-Rutishauser B (2012) Investigating the potential for different scooter and car exhaust emissions to cause cytotoxic and (pro-)inflammatory responses to a 3D in vitro model of the human epithelial airway. Toxicol Environ Chem 94(1):164–180. doi: 10.1080/02772248.2011.632509 CrossRefGoogle Scholar
- 32.Zhang HY, Ji ZX, Xia T, Meng H, Low-Kam C, Liu R, Pokhrel S, Lin SJ, Wang X, Liao YP, Wang MY, Li LJ, Rallo R, Damoiseaux R, Telesca D, Madler L, Cohen Y, Zink JI, Nel AE (2012) Use of metal oxide nanoparticle band gap to develop a predictive paradigm for oxidative stress and acute pulmonary inflammation. ACS Nano 6(5):4349–4368. doi: 10.1021/Nn3010087 CrossRefGoogle Scholar
- 33.Podechard N, Lecureur V, Le Ferrec E, Guenon I, Sparfel L, Gilot D, Gordon JR, Lagente V, Fardel O (2008) Interleukin-8 induction by the environmental contaminant benzo(a)pyrene is aryl hydrocarbon receptor-dependent and leads to lung inflammation. Toxicol Lett 177(2):130–137. doi: 10.1016/j.toxlet.2008.01.006 CrossRefGoogle Scholar
- 34.Umbuzeiro GA, Franco A, Martins MH, Kummrow F, Carvalho L, Schmeiser HH, Leykauf J, Stiborova M, Claxton LD (2008) Mutagenicity and DNA adduct formation of PAH, nitro-PAH, and oxy-PAH fractions of atmospheric particulate matter from Sao Paulo, Brazil. Mutat Res Genet Toxicol Environ 652(1):72–80. doi: 10.1016/j.mrgentox.2007.12.007 CrossRefGoogle Scholar
- 37.Lucking AJ, Lundback M, Barath SL, Mills NL, Sidhu MK, Langrish JP, Boon NA, Pourazar J, Badimon JJ, Gerlofs-Nijland ME, Cassee FR, Boman C, Donaldson K, Sandstrom T, Newby DE, Blomberg A (2011) Particle traps prevent adverse vascular and prothrombotic effects of diesel engine exhaust inhalation in men. Circulation 123(16):1721–U1766. doi: 10.1161/Circulationaha.110.987263 CrossRefGoogle Scholar
- 39.Westphal GA, Krahl J, Munack A, Rusche Y, Schroder O, Hallier E, Bruning T, Bunger J (2012) Mutagenicity of diesel engine exhaust is eliminated in the gas phase by an oxidation catalyst but only slightly reduced in the particle phase. Environ Sci Technol 46(11):6417–6424. doi: 10.1021/Es300399e CrossRefGoogle Scholar