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Analytical and Bioanalytical Chemistry

, Volume 406, Issue 27, pp 6827–6833 | Cite as

Detection of the food allergen celery via loop-mediated isothermal amplification technique

  • Celine Zahradnik
  • Roland Martzy
  • Robert L. Mach
  • Rudolf Krska
  • Andreas H. Farnleitner
  • Kurt BrunnerEmail author
Research Paper
Part of the following topical collections:
  1. Advanced Food Analysis

Abstract

Since 2005, celery and celery products have to be labeled according to Directive 2003/89/EC due to their allergenic potential. In order to provide a DNA-based, rapid and simple detection method suitable for high-throughput analysis, a loop-mediated isothermal amplification (LAMP) assay for the detection of celery (Apium graveolens) was developed. The assay was tested for specificity for celery since closely related species also hold food relevance. The limit of detection (LOD) for spiked food samples was found to be as low as 7.8 mg of dry celery powder per kilogram. An evaluation of different amplification and detection platforms was performed to show reliable detection independent from the instrument used for amplification (thermal cycler or heating block) and detection mechanisms (real-time fluorescence detection, agarose gel electrophoresis or nucleic acid staining). The analysis of 10 commercial food samples representing diverse and complex food matrices, and a false-negative rate of 0 % for approximately 24 target copies or 0.08 ng celery DNA for three selected food matrices show that LAMP has the potential to be used as an alternative strategy for the detection of allergenic celery. The performance of the developed LAMP assay turned out to be equal or superior to the best available PCR assay for the detection of celery in food products.

Keywords

Loop-mediated isothermal amplification Celery Food allergen Isothermal amplification Visual detection 

Notes

Acknowledgments

This work was financially supported by the Federal Country Lower Austria in cooperation with the European Regional Development Fund (ERDF).

Supplementary material

216_2014_7873_MOESM1_ESM.pdf (192 kb)
ESM 1 (PDF 191 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Celine Zahradnik
    • 1
  • Roland Martzy
    • 1
  • Robert L. Mach
    • 2
  • Rudolf Krska
    • 3
  • Andreas H. Farnleitner
    • 2
  • Kurt Brunner
    • 1
    Email author
  1. 1.Institute of Chemical Engineering, IFA-Tulln, Center for Analytical ChemistryVienna University of TechnologyTullnAustria
  2. 2.Institute of Chemical Engineering, Gene Technology GroupVienna University of TechnologyViennaAustria
  3. 3.Department IFA-Tulln, Center for Analytical ChemistryUniversity of Natural Resources and Applied Life Sciences ViennaTullnAustria

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