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A biocatalytic cascade with several output signals—towards biosensors with different levels of confidence

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Abstract

The biocatalytic cascade based on enzyme-catalyzed reactions activated by several biomolecular input signals and producing output signal after each reaction step was developed as an example of a logically reversible information processing system. The model system was designed to mimic the operation of concatenated AND logic gates with optically readable output signals generated at each step of the logic operation. Implications include concurrent bioanalyses and data interpretation for medical diagnostics.

A biocatalytic cascade with several inputs–outputs was designed for bioanalytical applications providing responses with increasing levels of confidence

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Acknowledgments

This work was supported by the NSF award #CBET-1066397 (Clarkson University) and by Grant No. EB014586 (Univ. of CT) from the National Institute of Biomedical Imaging and Bioengineering (NIBIB), NIH.

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Authors and Affiliations

  1. Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699-5810, USA

    Nataliia Guz & Evgeny Katz

  2. Department of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, NY, 12222, USA

    Jan Halámek

  3. Department of Chemistry and Institute of Materials Science, University of Connecticut, 55 N. Eagleville Rd., Storrs, CT, 06269-3060, USA

    James F. Rusling

  4. School of Chemistry, National University of Ireland, Galway, Ireland

    James F. Rusling

  5. Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, 06032, USA

    James F. Rusling

Authors
  1. Nataliia Guz
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  2. Jan Halámek
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  3. James F. Rusling
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  4. Evgeny Katz
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Corresponding author

Correspondence to Evgeny Katz.

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Guz, N., Halámek, J., Rusling, J.F. et al. A biocatalytic cascade with several output signals—towards biosensors with different levels of confidence. Anal Bioanal Chem 406, 3365–3370 (2014). https://doi.org/10.1007/s00216-014-7789-5

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  • DOI: https://doi.org/10.1007/s00216-014-7789-5

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