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Analytical and Bioanalytical Chemistry

, Volume 406, Issue 3, pp 695–704 | Cite as

Analysis of cyathane-type diterpenoids from Cyathus striatus and Hericium erinaceus by high-resolution MALDI MS imaging

  • Dhaka Ram Bhandari
  • Tian Shen
  • Andreas Römpp
  • Holger Zorn
  • Bernhard Spengler
Research Paper

Abstract

Fungal secondary metabolites in both fruiting bodies and pellets from submerged cultures of basidiomycetes were analyzed by atmospheric pressure matrix-assisted laser desorption/ionization-mass spectrometry imaging at a lateral resolution of 15 μm, a mass resolution of 140,000 at m/z 200 and a mass accuracy of better than 2 ppm. The striatals A, B, C, and D, and a number of erinacine type metabolites were detected in the basidiomycetes Cyathus striatus and Hericium erinaceus, respectively. The two fungi were selected as model species, as they are well-known for efficient production of terpenoid secondary metabolites with interesting biological activities, e.g., antibacterial, fungicidal, cytotoxic properties, and stimulating effects on nerve growth factor synthesis. The localization of metabolites revealed a mostly homogeneous distribution of the striatals in the pellets of C. striatus, while a concentration gradient, increasing to the center, was observed in the pellets of H. erinaceus. A mostly homogeneous distribution of metabolites was also found in the fruiting body of H. erinaceus.

Keywords

Foods/beverages Mass spectrometry imaging Fungi Secondary metabolites MALDI High resolution 

Notes

Acknowledgments

Financial support by the Hessian Ministry of Science and Art (LOEWE Research Focus ‘integrative fungal research’) and by the Deutsche Forschungsgemeinschaft (DFG, Sp 314/13-1) is gratefully acknowledged.

Supplementary material

216_2013_7496_MOESM1_ESM.pdf (295 kb)
ESM 1 (PDF 294 KB)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Dhaka Ram Bhandari
    • 1
  • Tian Shen
    • 2
  • Andreas Römpp
    • 1
  • Holger Zorn
    • 2
  • Bernhard Spengler
    • 1
  1. 1.Institute of Inorganic and Analytical ChemistryJustus Liebig University GiessenGiessenGermany
  2. 2.Institute of Food Chemistry and Food BiotechnologyJustus Liebig University GiessenGiessenGermany

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