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Enantioseparation of flinderoles and borreverines by HPLC on Chirobiotic V and V2 stationary phases and by CE using cyclodextrin selectors

Analytical and Bioanalytical Chemistry volume 405, pages 9169–9177 (2013)Cite this article

An Erratum to this article was published on 10 January 2014

Abstract

Racemic mixtures of the promising anti-malarial bisindole alkoids, flinderole A–C, desmethyl flinderole C, borreverine and isoborreverine, are baseline-separated for the first time by HPLC using vancomycin-based stationary phases and partially separated by capillary electrophoresis (CE) using cyclodextrin selectors. The HPLC results compare the performance of Chirobiotic V and V2 in the polar organic and reversed phase modes and their complementary selectivity is discussed. The performance of the cyclodextrin selectors in CE, while less effective, are discussed in terms of their selectivity in normal and reversed polarity modes.

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Acknowledgments

We gratefully acknowledge the Welch Foundation (Y-0026) for their continued support.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX, 76019, USA

    Jonathan P. Smuts & Daniel W. Armstrong

  2. Seoul Center, Korea Basic Science Institute, 136701, Seoul, South Korea

    Yun-Cheol Na

  3. Department of Chemistry, University of Houston, 136 Fleming Building, Houston, TX, 77204-5003, USA

    Ravikrishna Vallakati & Jeremy A. May

  4. Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Palacký University, 17. Listopadu 12, 77146, Olomouc, Czech Republic

    Adam Přibylka

Authors
  1. Jonathan P. Smuts
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  2. Yun-Cheol Na
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  3. Ravikrishna Vallakati
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  4. Adam Přibylka
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  5. Jeremy A. May
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  6. Daniel W. Armstrong
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Correspondence to Daniel W. Armstrong.

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Smuts, J.P., Na, YC., Vallakati, R. et al. Enantioseparation of flinderoles and borreverines by HPLC on Chirobiotic V and V2 stationary phases and by CE using cyclodextrin selectors. Anal Bioanal Chem 405, 9169–9177 (2013). https://doi.org/10.1007/s00216-013-7335-x

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  • DOI: https://doi.org/10.1007/s00216-013-7335-x

Keywords

  • Chirobiotic V
  • HPLC
  • Bisindole alkaloids
  • CE