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Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method

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Abstract

An improved online two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (2D LC-QToF MS) system was developed for the lipid profiling of human plasma, in which different lipid classes were separated by the first dimensional normal-phase (NP) LC and different lipid molecular species were separated by the second dimensional reversed-phase (RP) LC. This 2D LC-QToF MS system was built based on a ten-port, two-position valve as the interface, the conditions of which had been optimized and discussed in detail. As two loops were used to trap and transfer the first dimensional elute to the second dimension separately, this new interface suppressed the sample band broadening in the first dimensional column, increased the recovery and repeatability of 2D LC interface, and offered the possibility for the realization of not-stop-flow NP/RP 2D LC system. Finally, 190 endogenous lipid species out of 10 lipid classes were determined within a single run from the plasma of peritoneal patients. This method was also applied to identify the difference in lipid profile between plasma from peritoneal dialysis patients with bad volume status and peritoneal dialysis patients with good volume status. The discovery of 30 potential biomarkers would be helpful to the malnutrition, inflammation, and atherosclerosis syndrome investigation.

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Abbreviations

2D LC:

Two-dimensional liquid chromatography

EICs:

Extracted ion chromatograms

FFA:

Free fatty acids

LPC:

Lysoglycerophosphocholine

LPE:

Lysoglycerophosphoethanolamine

LPI:

Lysoglycerophosphoinositols

LPS:

Lysoglycerophosphoserine

MIA:

Malnutrition, inflammation and atherosclerosis syndrome

MS:

Mass spectrometry

NPLC:

Normal-phase liquid chromatography

PC:

Glycerophosphocholine

PCA:

Principal component analysis

PE:

Glycerophosphoethanolamine

PI:

Glycerophosphoinositol

PS:

Glycerophosphoserine

QToF:

Quadrupole time-of-flight mass spectrometry

RPLC:

Reversed-phase liquid chromatography

SM:

Sphingomyelin

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Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (grant no. 21175005). The authors wish to thank Mr. Chengsen Zhang for his kind help in drawing the figure of 2D LC interface.

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Authors and Affiliations

  1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China

    Min Li, Baosheng Feng, Yuan Liang, Wei Zhang, Yu Bai & Huwei Liu

  2. Division of Nephrology, Peking University Third Hospital, Beijing, 100191, China

    Wen Tang & Tao Wang

Authors
  1. Min Li
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  2. Baosheng Feng
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  3. Yuan Liang
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  4. Wei Zhang
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  5. Yu Bai
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  6. Wen Tang
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  7. Tao Wang
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  8. Huwei Liu
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Corresponding author

Correspondence to Huwei Liu.

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Li, M., Feng, B., Liang, Y. et al. Lipid profiling of human plasma from peritoneal dialysis patients using an improved 2D (NP/RP) LC-QToF MS method. Anal Bioanal Chem 405, 6629–6638 (2013). https://doi.org/10.1007/s00216-013-7109-5

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  • DOI: https://doi.org/10.1007/s00216-013-7109-5

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