Analytical and Bioanalytical Chemistry

, Volume 405, Issue 19, pp 6145–6154 | Cite as

Evaluation of diverse peptidyl motifs for cellular delivery of semiconductor quantum dots

  • Kelly Boeneman Gemmill
  • Markus Muttenthaler
  • James B. Delehanty
  • Michael H. Stewart
  • Kimihiro Susumu
  • Philip E. Dawson
  • Igor L. Medintz
Paper in Forefront
Part of the following topical collections:
  1. Optical Nanosensing in Cells

Abstract

Cell-penetrating peptides (CPPs) have rapidly become a mainstay technology for facilitating the delivery of a wide variety of nanomaterials to cells and tissues. Currently, the library of CPPs to choose from is still limited, with the HIV TAT-derived motif still being the most used. Among the many materials routinely delivered by CPPs, nanoparticles are of particular interest for a plethora of labeling, imaging, sensing, diagnostic, and therapeutic applications. The development of nanoparticle-based technologies for many of these uses will require access to a much larger number of functional peptide motifs that can both facilitate cellular delivery of different types of nanoparticles to cells and be used interchangeably in the presence of other peptides and proteins on the same surface. Here, we evaluate the utility of four peptidyl motifs for their ability to facilitate delivery of luminescent semiconductor quantum dots (QDs) in a model cell culture system. We find that an LAH4 motif, derived from a membrane-inserting antimicrobial peptide, and a chimeric sequence that combines a sweet arrow peptide with a portion originating from the superoxide dismutase enzyme provide effective cellular delivery of QDs. Interestingly, a derivative of the latter sequence lacking just a methyl group was found to be quite inefficient, suggesting that even small changes can have significant functional outcomes. Delivery was effected using 1 h incubation with cells, and fluorescent counterstaining strongly suggests an endosomal uptake process that requires a critical minimum number or ratio of peptides to be displayed on the QD surface. Concomitant cytoviability testing showed that the QD–peptide conjugates are minimally cytotoxic in the model COS-1 cell line tested. Potential applications of these peptides in the context of cellular delivery of nanoparticles and a variety of other (bio)molecules are discussed.

Keywords

Cell-penetrating peptide Quantum dot Nanoparticle Cell Delivery Endocytosis Labeling Nanotechnology Sweet arrow peptide Quantum dot Metal affinity 

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Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2013

Authors and Affiliations

  • Kelly Boeneman Gemmill
    • 1
  • Markus Muttenthaler
    • 3
  • James B. Delehanty
    • 1
  • Michael H. Stewart
    • 2
  • Kimihiro Susumu
    • 2
    • 4
  • Philip E. Dawson
    • 3
  • Igor L. Medintz
    • 1
  1. 1.Center for Bio/Molecular Science and Engineering Code 6900U.S. Naval Research LaboratoryWashingtonUSA
  2. 2.Division of Optical Sciences Code 5600U.S. Naval Research LaboratoryWashingtonUSA
  3. 3.Departments of Chemistry and Cell BiologyThe Scripps Research InstituteLa JollaUSA
  4. 4.Sotera Defense SolutionsAnnapolis JunctionUSA

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