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A fast and simple procedure for determination of perfluoroalkyl substances in food and feed: a method verification by an interlaboratory study


In this study, a simple, fast, and cheap sample preparation procedure for the analysis of three well-known representatives of perfluoroalkyl substances (perfluorooctane sulfonate, perfluorooctanoic acid, and perfluorooctane sulfonamide) was validated in accordance with Commission Decision 2002/657/EC. The method was based on extraction with methanol followed by a dispersive solid phase extraction cleanup step by addition of activated charcoal for fish tissue, fish feed, and milk samples. The novel analytical approach combined with liquid chromatography–tandem mass spectrometry makes it possible to achieve limits of quantification below 1 μg/kg (defined by Commission Recommendation 2010/161/EU). This method provides a high laboratory sample throughput: ten samples in 60 min. The validated procedure was successfully verified in an interlaboratory study.

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This study was funded by the FP7 European project CONffIDENCE “Contaminants in food and feed: Inexpensive detection for control of exposure” (no. 211326) and by the projects MSM 6046137305 and IDS 7E08068 supported by the Ministry of Education, Youth and Sports of the Czech Republic.

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Correspondence to J. Poustka.

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Published in the topical collection Rapid Detection in Food and Feed with guest editors Rudolf Krska and Michel Nielen.

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Hrádková, P., Poustka, J., Pulkrabová, J. et al. A fast and simple procedure for determination of perfluoroalkyl substances in food and feed: a method verification by an interlaboratory study. Anal Bioanal Chem 405, 7817–7827 (2013).

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  • Perfluoroalkyl substances
  • Activated charcoal
  • Liquid chromatography–tandem mass spectrometry
  • Method validation
  • Commission Decision 2002/657/EC
  • Commission Recommendation 2010/161/EU