Analytical and Bioanalytical Chemistry

, Volume 405, Issue 16, pp 5381–5386 | Cite as

Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing

  • Stefan Jezierski
  • Anke S. Klein
  • Christian Benz
  • Michael Schaefer
  • Stefan Nagl
  • Detlev Belder
Rapid Communication


We immobilized adherent human embryonic kidney (HEK) cells—which are able to trace adenosine triphosphate (ATP) —inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20 μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds.


Free-flow electrophoresis Adenosine triphosphate Microfluidic Cell response 

Supplementary material

216_2013_6945_MOESM1_ESM.pdf (935 kb)
ESM 1 (PDF 934 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Stefan Jezierski
    • 1
  • Anke S. Klein
    • 2
  • Christian Benz
    • 1
  • Michael Schaefer
    • 2
  • Stefan Nagl
    • 1
  • Detlev Belder
    • 1
  1. 1.Institut für Analytische ChemieUniversität LeipzigLeipzigGermany
  2. 2.Rudolf-Boehm-Institut für Pharmakologie und ToxikologieUniversität LeipzigLeipzigGermany

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