Advertisement

Analytical and Bioanalytical Chemistry

, Volume 405, Issue 16, pp 5381–5386 | Cite as

Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing

  • Stefan Jezierski
  • Anke S. Klein
  • Christian Benz
  • Michael Schaefer
  • Stefan Nagl
  • Detlev Belder
Rapid Communication

Abstract

We immobilized adherent human embryonic kidney (HEK) cells—which are able to trace adenosine triphosphate (ATP) —inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20 μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds.

Keywords

Free-flow electrophoresis Adenosine triphosphate Microfluidic Cell response 

Supplementary material

216_2013_6945_MOESM1_ESM.pdf (935 kb)
ESM 1 (PDF 934 kb)

References

  1. 1.
    Eltzov E, Marks RS (2011) Anal Bioanal Chem 400:895–913CrossRefGoogle Scholar
  2. 2.
    Harms H, Wells MC, van der Meer JR (2006) App Microbiol Biotechnol 70:273–280CrossRefGoogle Scholar
  3. 3.
    Foudeh AM, Fatanat Didar T, Veres T, Tabrizian M (2012) Lab Chip 12:3249–3266CrossRefGoogle Scholar
  4. 4.
    Keusgen M (2002) Naturwissenschaften 89:433–444CrossRefGoogle Scholar
  5. 5.
    Raymond DE, Manz A, Widmer HM (1994) Anal Chem 66:2858–2865CrossRefGoogle Scholar
  6. 6.
    Kohlheyer D, Eijkel JCT, van den Berg A, Schasfoort RBM (2008) Electrophoresis 29:977–993CrossRefGoogle Scholar
  7. 7.
    Kostal V, Fonslow BR, Arriaga EA, Bowser MT (2009) Anal Chem 81:9267–9273CrossRefGoogle Scholar
  8. 8.
    Podszun S, Vulto P, Heinz H, Hakenberg S, Hermann C, Hankemeier T, Urban GA (2012) Lab Chip 12:451–457CrossRefGoogle Scholar
  9. 9.
    Köhler S, Nagl S, Fritzsche S, Belder D (2012) Lab Chip 12:458–463CrossRefGoogle Scholar
  10. 10.
    Becker M, Budich C, Deckert V, Janasek D (2009) Analyst 134:38–40CrossRefGoogle Scholar
  11. 11.
    Jezierski S, Belder D, Nagl S (2013) Chem Comm 49:904–906CrossRefGoogle Scholar
  12. 12.
    Oja SM, Wood M, Zhang B (2013) Anal Chem 85:473–486CrossRefGoogle Scholar
  13. 13.
    Steude A, Schmidt S, Robitzki AA, Pänke O (2011) Lab Chip 11:2884–2892CrossRefGoogle Scholar
  14. 14.
    Richter L, Stepper C, Mak A, Reinthaler A, Heer R, Kast M, Brückl H, Ertl P (2007) Lab Chip 7:1723–1731CrossRefGoogle Scholar
  15. 15.
    Asphahani F, Zhang M (2007) Analyst 132:835–841CrossRefGoogle Scholar
  16. 16.
    Köhler S, Benz C, Becker H, Beckert E, Beushausen V, Belder D (2012) RSC Adv 1:520–525CrossRefGoogle Scholar
  17. 17.
    Schneider CA, Rasband WS, Eliceiri KW (2012) Nat Methods 9:671–675CrossRefGoogle Scholar
  18. 18.
    Graham FL, Smiley J, Russel WC, Nairn R (1977) J Gen Virol 36:59–72CrossRefGoogle Scholar
  19. 19.
    Fischer W, Franke H, Groger-Arndt H, Illes P (2005) Naunyn-Schmiedeberg’s Arch Pharmacol 371:466–472CrossRefGoogle Scholar
  20. 20.
    Bondy B, Bauer J, Seuffert I, Weber G (1995) Electrophoresis 16:92–97CrossRefGoogle Scholar
  21. 21.
    Wang F, Wang H, Wang J, Wang H-Y, Rummel PL, Garimella SV, Chang L (2008) Biotechnol Bioeng 100:150–158CrossRefGoogle Scholar
  22. 22.
    North RA (2002) Physiol Rev 82:1013–1067Google Scholar
  23. 23.
    Guerini D, Coletto L, Carafoli E (2005) Cell Calcium 38:281–289CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Stefan Jezierski
    • 1
  • Anke S. Klein
    • 2
  • Christian Benz
    • 1
  • Michael Schaefer
    • 2
  • Stefan Nagl
    • 1
  • Detlev Belder
    • 1
  1. 1.Institut für Analytische ChemieUniversität LeipzigLeipzigGermany
  2. 2.Rudolf-Boehm-Institut für Pharmakologie und ToxikologieUniversität LeipzigLeipzigGermany

Personalised recommendations