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Repeat MALDI MS imaging of a single tissue section using multiple matrices and tissue washes

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Abstract

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) techniques are continually being assessed with a view to improving the quality of information obtained from a given sample. A single tissue section will typically only be analyzed once by MALDI MSI and is then either used for histological staining or discarded. In this study, we explore the idea of repeat analysis of a single tissue section by MALDI MSI as a route toward improving sensitivity, structural characterization, and diversity of detected analyte classes. Repeat analysis of a single tissue section from a fresh frozen mouse brain is investigated with both α-cyano-4-hydroxycinnamic acid (CHCA) and para-nitroaniline (PNA). Repeat analysis is then applied to the acquisition of MALDI MSI and MALDI tandem mass spectrometry imaging employing collision induced dissociation (MS/MS imaging employing CID) from a formalin-fixed mouse brain section. Finally, both lipid and protein data are acquired from the same tissue section via repeat analysis utilizing CHCA, sinapinic acid (SA), and a tissue wash step. PNA was found to outperform CHCA as a matrix for repeat analysis; multiple lipids were identified using MS/MS imaging; both lipid and protein images were successfully acquired from a single tissue section.

Repeat analysis by MALDI MS imaging of a single tissue section is investigated with multiple matrices and tissue washes to provide increased molecular information from a single tissue section

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Acknowledgments

The authors would like to thank the EPSRC for funding via studentships for R.T.S. through the PSIBS Doctoral Training Centre at the University of Birmingham (EP/F50053X/1).

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Authors and Affiliations

  1. PSIBS Centre, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK

    Rory T. Steven & Josephine Bunch

  2. School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK

    Rory T. Steven & Josephine Bunch

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  1. Rory T. Steven
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  2. Josephine Bunch
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Correspondence to Josephine Bunch.

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Steven, R.T., Bunch, J. Repeat MALDI MS imaging of a single tissue section using multiple matrices and tissue washes. Anal Bioanal Chem 405, 4719–4728 (2013). https://doi.org/10.1007/s00216-013-6899-9

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  • DOI: https://doi.org/10.1007/s00216-013-6899-9

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