Abstract
We report loading of vitamin C (ascorbic acid) on to lysozyme-shelled microbubbles. The interaction between lysozyme-shelled microbubbles and vitamin C was studied by use of cyclic and differential pulse voltammetry, zeta potential measurements, and scanning electron microscopy. The effect of microbubbles on electrochemical measurement of ascorbic acid was evaluated. The linear range for ascorbic acid obtained for differential pulse measurement in the presence of 1 mg mL−1 microbubbles was 1–50 μmol L−1 (y = 0.067x + 0.130, r 2 = 0.995), with a detection limit of 0.5 μmol L−1. The experimental conditions, i.e., pH and ionic strength, were optimized to improve the interaction between ascorbic acid and lysozyme-shelled microbubbles. The results were satisfactory when the interaction was performed for 1 h in aqueous solution at pH 6. The amount of vitamin C loaded on the microbubbles (90 % of the analyte added, RSD inter-expt. = 3 %, n = 6) and the stability of microbubbles–ascorbic acid complex (until 72 h at 25 °C) were also evaluated by use of differential pulse voltammetry and zeta potential measurements.
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References
Misra R, Acharya S, Sahoo SK (2010) Drug Discov Today 15(19–20):842–850
Sirsi S, Borden M (2009) Bubble Sci Eng Technol 1(1–2):3–17
Cavalieri F, Zhou M, Tortora M, Baldassarri L, Ashokkumar M (2012) Curr Pharm Des 18(15):2135–2151
Blomley MJ, Albrecht T, Cosgrove DO, Patel N, Jayaram V, Butler-Barnes J, Eckersley RJ, Bauer A, Schlief R (1999) Radiol 210:409–416
Hongjia Z, Rong X, Qiufang O, Lidian C, Baowei D, Ye H (2010) Eur J Radiol 73(2):288–293
Pitt WG, Husseini GA, Staples BJ (2004) Expert Opin Drug Deliv 1:37–56
Zhou M, Cavalieri F, Caruso F, Ashokkumar M (2012) ACS Macro Lett 1(7):853–856
Cavalieri F, Zhou M, Caruso F, Ashokkumar M (2011) Chem Commun 47:4096–4098
Melino S, Zhou M, Tortora M, Paci M, Cavalieri F, Ashokkumar M (2012) Amino Acids 43(2):885–896
Zhou M, Cavalieri F, Ashokkumar M (2011) Soft Matter 7:623–630
Englard S, Seifter S (1986) Annu Rev Nutr 6:365–406
Mak S, Egri Z, Tanna G, Coleman R, Newton GE (2002) Am J Physiol Heart Circ Physiol 282:H2414–H2421
May JM (1999) FASEB J 13:995–1006
Wilson JX (2009) Biofactors 35(1):5–13
Matos RC, Augelli MA, Pedrotti JJ, Lago CL, Angnes L (1998) Electroanalysis 10:653–658
Wring SA, Hart JP, Brich BJ (1990) Anal Chim Acta 229:63–70
Pandy NK (1982) Anal Chem 54:793–796
Erdurak-Kiliç CS, Uslu B, Dogan B, Ozgen U, Ozkan SA, Coskun M (2006) J Anal Chem 61:1113–1120
Tiwari KK (2010) J Chin Chem Soc 57:105–110
Wang Z, Liu J, Liang Q, Wang Y, Luo G (2002) Analyst 127:653–658
Bradford MM (1976) Anal Biochem 72:248–254
Sorensen K, Brodbech U (1986) J Immunol Meth 95:291–293
Addis T, Poo LJ, Lew W, Yuen DW (1936) J Biol Chem 115:111–116
Nomura H, Ishiro T, Morimoto S (1969) Chem Pharm Bull 17:387–393
Ou S, Kwok KC, Wang Y, Bao H (2004) Food Chem 88:317–320
Richards PG, Cloes B, Heptinstall J, Walton DJ (1994) Enzyme Microb Technol 16:795–801
Brabec V, Schindlerovà I (1981) Electrochem Bioelectrochem Bioenerg 8:451–458
Humble AV, Gado GM, Codd GA (1997) Water Res 7:1679–1686
Grieshaber D, MacKenzie R, Vörös J, Reimhult E (2008) Sensors 8:1400–1458
Wilson RJ, Breezer AE, Mitchell JC (1995) Thermochim Acta 27:27–40
Lee E, Kim K, Choi M, Lee Y, Park J, Kim B (2010) Drug Deliv 17(8):573–580
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This work was funded by the Marie Curie IRSES 7° FP European Project 2009-2010 and supported by the University of Melbourne IPRS fellowship and Vic Govt. Virs fellowship.
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Cavalieri, F., Micheli, L., Zhou, M. et al. Electrochemical investigation of the interaction between lysozyme-shelled microbubbles and vitamin C. Anal Bioanal Chem 405, 5531–5538 (2013). https://doi.org/10.1007/s00216-013-6895-0
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DOI: https://doi.org/10.1007/s00216-013-6895-0