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Preparation techniques alter the mineral and organic fractions of fish otoliths: insights using Raman micro-spectrometry

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Abstract

The high spatial resolution analysis of the mineral and organic composition of otoliths using Raman micro-spectrometry involves rigorous protocols for sample preparation previously established for microchemistry and trace elements analyses. These protocols often include otolith embedding in chemically neutral resin (i.e., resins which do not contain, in detectable concentration, elements usually sought in the otoliths). Such embedding may however induce organic contamination. In this paper, Raman micro-spectrometry reveals the presence of organic contamination onto the surface obtained from the use of epoxy resin, specifically Araldite. This contamination level varies depending on otolith structures. Core and checks, known as structural discontinuities, exhibit the most important level of contaminations. Our results suggest that otolith embedding with resin affects the organic matrix of the otolith, probably through an infiltration of the resin in the crystalline structure. The interpretation of chemical otolith signatures, especially Raman otolith signatures, and stable isotope analyses should then be revised in light of these results. In this respect, we propose a method for the correction of Raman otolith signatures for contamination effects.

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Authors and Affiliations

  1. Département des Sciences et Technologies Halieutiques, Ifremer, Z.I. Pointe du diable, CS 10070, 29280, Plouzané, France

    Aurélie Jolivet & Hélène de Pontual

  2. Institut Universitaire Européen de la Mer, Laboratoire des Sciences de L’Environnement Marin (UMR 6539), Université de Bretagne Occidentale, Technopôle Brest Iroise, Place Nicolas Copernic, 29280, Plouzané, France

    Aurélie Jolivet

  3. UMR 3192 LabSTICC, Institut Telecom/Telecom Bretagne, Technopole Brest Iroise, CS83818, 29238, Brest Cedex 03, France

    Ronan Fablet

  4. Université Européenne de Bretagne, 5 Boulevard Laënnec, 35000, Rennes, France

    Ronan Fablet

  5. Institut des Molécules et Matériaux du Mans (UMR CNRS 6283), LUNAM Université, Avenue Olivier Messiaen, 72085, Le Mans Cedex 09, France

    Jean-François Bardeau

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  1. Aurélie Jolivet
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  2. Ronan Fablet
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  4. Hélène de Pontual
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Correspondence to Aurélie Jolivet.

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Jolivet, A., Fablet, R., Bardeau, JF. et al. Preparation techniques alter the mineral and organic fractions of fish otoliths: insights using Raman micro-spectrometry. Anal Bioanal Chem 405, 4787–4798 (2013). https://doi.org/10.1007/s00216-013-6893-2

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  • DOI: https://doi.org/10.1007/s00216-013-6893-2

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