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Selective isolation of hemoglobin by use of imidazolium-modified polystyrene as extractant

Analytical and Bioanalytical Chemistry volume 405, pages 5353–5358 (2013)Cite this article

Abstract

Ionic liquids have attracted much attention in the analysis of a variety of species. The functional groups in ionic liquids can result in highly efficient separation and enrichment and, because of their typical lack of volatility, they are environmentally benign. We grafted imidazole cations onto the surface of chloromethyl polystyrene, denoted PS-CH2-[MIM]+Cl−, and this modified polymer was used to selectively extract the protein hemoglobin (Hb). The prepared extractant PS-CH2-[MIM]+Cl−, containing 2 mmol immobilized imidazole groups per gram polymer, was characterized by FT-IR, surface charge analysis, and elemental analysis. The adsorption efficiency was 91 %. The adsorption capacity of the PS-CH2-[MIM]+Cl− for Hb was 23.6 μg mg−1, and 80 % of the retained Hb could be readily recovered by use of 0.5 % (m/v) aqueous sodium dodecyl sulfate (SDS) solution as eluate. The activity of the eluted Hb was approximately 90 %. The prepared imidazole-containing solid phase polymer was used for direct adsorption of Hb without use of any other solid matrix as support of the ionic liquid. The material was used in practice to isolate Hb from human whole blood.

Coordination interaction between heme of hemoglobin and imidazolium-modified chloromethyl polystyrene.

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Acknowledgments

The authors are grateful for financial support from the Natural Science Foundation of China (51172039) and the Fundamental Research Funds for the Central Universities of China (N110805001).

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Affiliations

  1. Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China

    Gang Zhao & Rong-Huan He

  2. Research Center for Analytical Sciences, Northeastern University, Shenyang, 110819, China

    Shuai Chen & Xu-Wei Chen

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  1. Gang Zhao
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  2. Shuai Chen
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  3. Xu-Wei Chen
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Correspondence to Rong-Huan He.

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Zhao, G., Chen, S., Chen, XW. et al. Selective isolation of hemoglobin by use of imidazolium-modified polystyrene as extractant. Anal Bioanal Chem 405, 5353–5358 (2013). https://doi.org/10.1007/s00216-013-6889-y

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  • DOI: https://doi.org/10.1007/s00216-013-6889-y

Keywords

  • Ionic liquid immobilization
  • Protein
  • Adsorption
  • Hemoglobin
  • Biocompatibility
  • Solid-phase extraction