Metabolomics for unknown plant metabolites
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In this article we discuss current trends in the techniques available for plant metabolomics. Chemical assignment of unknown metabolites leads to understanding of biosynthetic mechanisms at the gene level for genome-sequenced plants. Metabolomics using mass spectrometry has achieved innovative results in phytochemical genomics for primary and secondary metabolism in the model plant Arabidopsis thaliana by using publicly and commercially available information and standard compounds. However, finding a consolidated analytical technique for elucidation of structural information (e.g., elemental composition and structure) remains challenging. Recently, hyphenated analytical techniques and computer-assisted structural analysis with high-throughput and high-accuracy have been developing. Metabolite-driven approaches using such technology will be of central importance in phytochemical genomics.
KeywordsMetabolomics Unknown secondary metabolites LC–MS LC–SPE–NMR–MS Computer-assisted structure elucidation
We would like to thank Dr Kazunori Saito, Dr Ulrich Braumann, and Dr Aiko Barsch (Bruker Daltonics) for the pictures of FT–ICR–MS and LC–SPE–NMR–MS. We also thank Ms Yumiko Iizuka for the illustrations. This study was supported by JST, Strategic International Research Cooperative Program (SICP), JST, Strategic International Collaborative Research Program (SICORP), and Japan Advanced Plant Science Network. Finally, we extend our apologies to authors whose work has not been cited because of the limited space available.
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