Analytical and Bioanalytical Chemistry

, Volume 405, Issue 14, pp 4959–4962 | Cite as

“Do it yourself” reference materials for δ13C determinations by isotope ratio mass spectrometry

Note

Abstract

A method is described to prepare inexpensive organic reference materials for routine stable isotope work. The method blends isotope-enriched and isotope-depleted materials to match closely international measurement standards. Examples are presented showing how 13C-enriched and 13C-depleted glucose were blended to match the isotopic compositions of the international carbon isotope standards, NBS 19 and L-SVEC. Preparation of the blended standards was straightforward, and the prepared solutions proved easy to dispense into tin capsules and easy to combust in a conventional isotope analysis system. The blended materials allow a laboratory to perform a two-point calibration for isotopic composition on a daily basis, with the use of an international reference material recommended for routine quality assurance. The blending method can be applied widely to make isotope standards for other elements and compounds.

Keywords

Stable isotope ratio measurements Isotope ratio mass spectrometry Reference materials Quality assurance 

Supplementary material

216_2013_6851_MOESM1_ESM.pdf (638 kb)
ESM 1(PDF 638 KB)

References

  1. 1.
    Qi H, Coplen TB, Geilmann H, Brand WA, Böhlke JK (2003) Two new organic reference materials for δ 13C and δ 15N measurements and a new value for the δ 13C of NBS 22 oil. Rapid Commun Mass Spectrom 17(22):2483–2487. doi:10.1002/rcm.1219 CrossRefGoogle Scholar
  2. 2.
    Coplen TB, Brand WA, Gehre M, Gröning M, Meijer HAJ, Toman B, Verkouteren RM (2006) New guidelines for δ 13C measurements. Anal Chem 78(7):2439–2441. doi:10.1021/ac052027c CrossRefGoogle Scholar
  3. 3.
    Skrzypek G, Sadler R, Paul D (2011) Error propagation in normalization of stable isotope data: a Monte Carlo analysis. Rapid Commun Mass Spectrom 24(18):2697–2705. doi:10.1002/rcm.4684 CrossRefGoogle Scholar
  4. 4.
    Coplen TB, Bohlke JK, DeBievre P, Ding T, Holden NE, Hopple JA, Krouse HR, Lamberty A, Peiser HS, Revesz K, Rieder SE, Rosman KJR, Roth E, Taylor PDP, Vocke RD Jr, Xiao YK (2002) Isotope-abundance variations of selected elements (IUPAC Technical Report). Pure Appl Chem 74(10):1987–2017CrossRefGoogle Scholar
  5. 5.
    David GE, Coxon A, Frewa RD, Hayman AR (2010) Isotope fractionation during precipitation of methamphetamine HCl and discrimination of seized forensic samples. Forensic Sci Int 200(1-3):123–129. doi:10.1016/j.forsciint.2010.03.043 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Queensland Health Forensic and Scientific ServiceBrisbaneAustralia
  2. 2.Griffith University - Australian Rivers InstituteBrisbaneAustralia

Personalised recommendations