Molecular cartography in acute Chlamydia pneumoniae infections—a non-targeted metabolomics approach

Analytical and Bioanalytical Chemistry volume 405, pages5119–5131(2013)Cite this article

Abstract

Infections with Chlamydia pneumoniae cause several respiratory diseases, such as community-acquired pneumonia, bronchitis or sinusitis. Here, we present an integrated non-targeted metabolomics analysis applying ultra-high-resolution mass spectrometry and ultra-performance liquid chromatography mass spectrometry to determine metabolite alterations in C. pneumoniae-infected HEp-2 cells. Most important permutations are elaborated using uni- and multivariate statistical analysis, logD retention time regression and mass defect-based network analysis. Classes of metabolites showing high variations upon infection are lipids, carbohydrates and amino acids. Moreover, we observed several non-annotated compounds as predominantly abundant after infection, which are promising biomarker candidates for drug-target and diagnostic research.

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Abbreviations

DI:

Direct injection

EB:

Elementary bodies

ESI:

Electrospray ionization

FCS:

Fetal calf serum

GSH:

Glutathione

GSSG:

Oxidized glutathione

HILIC:

Hydrophilic interaction liquid chromatography

ICR/FT-MS:

Ion cyclotron resonance Fourier transform mass spectrometry

m/z :

Mass/charge

NDP:

Nucleoside-diphosphate

NEAA:

Non-essential amino acids

NTP:

Nucleoside-triphosphate

PCA:

Principal component analysis

PLS-DA:

Partial least square discriminative analysis

RP:

Reversed phase

S/N:

Signal-to-noise ratio

UPLC:

Ultra-performance liquid chromatography

VIP:

Variable importance in projection

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Acknowledgments

This work received financial support from the ERA-NET PathoGenoMics ‘Pathomics’ (0315442C).

Author information

Affiliations

  1. Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764, Neuherberg, Germany

    Constanze Müller, Dimitrios Tziotis, Franco Moritz & Philippe Schmitt-Kopplin

  2. Institute for Medical Microbiology and Hygiene, University of Lübeck, 23538, Lübeck, Germany

    Inga Dietz & Jan Rupp

  3. Medical Clinic III/ Infectious Diseases, University Hospital of Schleswig-Holstein, Campus Lübeck, 23562, Lübeck, Germany

    Jan Rupp

  4. Chair of Analytical Food Chemistry, Technical University of Munich, 80333, Freising, Germany

    Philippe Schmitt-Kopplin

Authors
  1. Constanze Müller
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  2. Inga Dietz
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  3. Dimitrios Tziotis
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  4. Franco Moritz
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  5. Jan Rupp
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  6. Philippe Schmitt-Kopplin
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Corresponding author

Correspondence to Philippe Schmitt-Kopplin.

Additional information

Published in the topical collection Metabolomics and Metabolite Profiling with guest editors Rainer Schuhmacher, Rudolf Krska, Roy Goodacre, and Wolfram Weckwerth.

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Müller, C., Dietz, I., Tziotis, D. et al. Molecular cartography in acute Chlamydia pneumoniae infections—a non-targeted metabolomics approach. Anal Bioanal Chem 405, 5119–5131 (2013). https://doi.org/10.1007/s00216-013-6732-5

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Keywords

  • Mass spectrometry
  • Host–pathogen interactions
  • Liquid chromatography