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Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modified nanoporous gold electrodes

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Abstract

Nanoporous and planar gold electrodes were utilised as supports for the redox enzymes Aspergillus niger glucose oxidase (GOx) and Corynascus thermophilus cellobiose dehydrogenase (CtCDH). Electrodes modified with hydrogels containing enzyme, Os-redox polymers and the cross-linking agent poly(ethylene glycol)diglycidyl ether were used as biosensors for the determination of glucose and lactose. Limits of detection of 6.0 (±0.4), 16.0 (±0.1) and 2.0 (±0.1) μM were obtained for CtCDH-modified lactose and glucose biosensors and GOx-modified glucose biosensors, respectively, at nanoporous gold electrodes. Biofuel cells composed of GOx- and CtCDH-modified gold electrodes were utilised as anodes, together with Myrothecium verrucaria bilirubin oxidase (MvBOD) or Melanocarpus albomyces laccase as cathodes, in biofuel cells. A maximum power density of 41 μW/cm2 was obtained for a CtCDH/MvBOD biofuel cell in 5 mM lactose and O2-saturated buffer (pH 7.4, 0.1 M phosphate, 150 mM NaCl).

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Acknowledgements

This work was supported by the European Union FP7 project, 3D-nanobiodevices (NMP4-SL-2009-229255) and the Programme for Third Level Institutions funded nanoscience programme, INSPIRE.

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Authors and Affiliations

  1. Department of Chemical and Environmental Sciences, Materials and Surface Science Institute, University of Limerick, Limerick, Ireland

    Urszula Salaj-Kosla, Micheál D. Scanlon, Tobias Baumeister & Edmond Magner

  2. Department of Food Science and Technology, Food Biotechnology Laboratory, University of Natural Resources and Applied Life Sciences, 1180, Vienna, Austria

    Kawah Zahma & Roland Ludwig

  3. School of Chemistry & Ryan Institute, National University of Ireland Galway, Galway, Ireland

    Peter Ó Conghaile, Domhnall MacAodha & Dónal Leech

Authors
  1. Urszula Salaj-Kosla
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  2. Micheál D. Scanlon
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  3. Tobias Baumeister
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  4. Kawah Zahma
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  5. Roland Ludwig
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  6. Peter Ó Conghaile
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  7. Domhnall MacAodha
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  8. Dónal Leech
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  9. Edmond Magner
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Corresponding author

Correspondence to Edmond Magner.

Additional information

Published in the topical collection Bioelectroanalysis with guest editors Nicolas Plumeré, Magdalena Gebala, and Wolfgang Schuhmann.

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Salaj-Kosla, U., Scanlon, M.D., Baumeister, T. et al. Mediated electron transfer of cellobiose dehydrogenase and glucose oxidase at osmium polymer-modified nanoporous gold electrodes. Anal Bioanal Chem 405, 3823–3830 (2013). https://doi.org/10.1007/s00216-012-6657-4

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  • DOI: https://doi.org/10.1007/s00216-012-6657-4

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