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Comparison of different extraction methods for simultaneous determination of B complex vitamins in nutritional yeast using LC/MS-TOF and stable isotope dilution assay

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Abstract

The application of LC/MS-TOF method combined with stable isotope dilution assay was studied for determination of thiamine, riboflavin, nicotinamide, nicotinic acid, pantothenic acid, pyridoxal, and pyridoxine in food. Nutritional yeast powder was used as a model food matrix. Acid extraction was compared with various enzymatic treatments in ammonium formate buffer to find a suitable method for the conversion of more complex vitamers into the same forms as the used isotope-labeled internal standards. The enzyme preparations α-amylase, takadiastase, β-glucosidase, and acid phosphatase were all able to liberate thiamine and riboflavin. The diastatic enzyme preparations α-amylase and takadiastase also expressed proteolytic side activities resulting in the formation of small peptides which interfered with the mass spectra of thiamine and riboflavin. Liberation of nicotinamide and pantothenic acid from NAD+ and CoA, respectively, could not be achieved with any of the studied enzyme preparations. Hydrochloric acid extraction at 121 °C for 30 min was found to be destructive to pantothenic acid, but increased the liberation of pyridoxal.

Comparison of different extraction methods for B complex vitamins determination in nutritional yeast

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Acknowledgments

The financial support for this research was provided by the European Regional Development Fund project EU29994, Estonian Ministry of Education and Research grant SF0140090s08, and by Estonian Science Foundation grant G7323. We would like to thank David Schryer for revision of the manuscript.

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Authors and Affiliations

  1. Department of Food Processing, Tallinn University of Technology, Ehitajate tee 5, 19086, Tallinn, Estonia

    Kristel Hälvin, Toomas Paalme & Ildar Nisamedtinov

  2. Competence Centre of Food and Fermentation Technologies, Akadeemia tee 15a, 12618, Tallinn, Estonia

    Kristel Hälvin & Ildar Nisamedtinov

  3. Lallemand, Inc., 5494 Notre-Dame East, Montréal, QC, H1N 2C4, Canada

    Ildar Nisamedtinov

Authors
  1. Kristel Hälvin
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  2. Toomas Paalme
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Correspondence to Kristel Hälvin.

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Hälvin, K., Paalme, T. & Nisamedtinov, I. Comparison of different extraction methods for simultaneous determination of B complex vitamins in nutritional yeast using LC/MS-TOF and stable isotope dilution assay. Anal Bioanal Chem 405, 1213–1222 (2013). https://doi.org/10.1007/s00216-012-6538-x

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  • DOI: https://doi.org/10.1007/s00216-012-6538-x

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