Abstract
Liquid chromatography negative ion electrospray ionisation tandem mass spectrometry has been used for characterisation of naturally occurring prenylated fungal metabolites and synthetic derivatives. The fragmentation studies allow an elucidation of the decomposition pathways for these compounds. It could be shown, that the prenyl side chain is degraded by successive radical losses of C5 units. Both the benzoquinones and the phenolic derivatives display significant key ions comprising the aromatic ring. In some cases, the formation of significant oxygen-free key ions could be evidenced by high-resolution MS/MS measurements. Furthermore, the different types of basic skeletons, benzoquinones and phenol type as well as cyclic prenylated compounds, can be differentiated by their MS/MS behaviour.
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References
Cornforth JW (1968) Terpenoid biosynthesis. Chem Ber 4:102–106
Geris R, Simpson TJ (2009) Meroterpenoids produced by fungi. Nat Prod Rep 26:1063–1094
Simpson TJ (1987) Applications of multinuclear NMR to structural and biosynthetic studies of polyketide microbial metabolites. Chem Soc Rev 16:123–160
Gill M, Steglich W (1987) Pigments of fungi (Macromycetes). In: Zechmeister L, Herz W, Grisebach H, Kirby GW, Tamm CH (eds) Progress in the chemistry of organic natural products, vol. 51. Springer, Wien
Beaumont PC, Edwards RL (1969) Constituents of the higher fungi. Part IX. Bovinone, 2,5-dihydroxy-3-geranylgeranyl-1,4-benzoquinone from Boletus (Suillus) bovinus (Linn. ex Fr.) Kuntze. J Chem Soc Perkin 1 18:2398–2403
Beaumont PC, Edwards RL (1971) Constituents of the higher fungi. Part XI. Boviquinone-3, (2,5-dihydroxy-3-farnesyl-1,4-benzoquinone), diboviquinone-3,4, methylenediboviquinone-3,3, and xerocomic acid from Gomphidius rutilus Fr. and diboviquinone-4,4 from Boletus (Suillus) bovinus (Linn. ex Fr.) Kuntze. J Chem Soc Perkin 1 14:2582–2585
Steglich W, Esser F, Pils I (1971) Helveticon, ein Benzochinon-Derivat vom Bovinon-Typ aus Chroogomphus helveticus und Ch. rutilus. Z. Naturforsch 26b:336
Besl H, Hecht HJ, Luger P, Pasupathy V, Steglich W (1975) Pilzpigmente, XXIII. Tridentochinon, ein [13](3,6)Benzofuranophan aus Suillus tridentinus (Boletales). Chem Ber 108:3675–3691
Jägers E, Pasupathy V, Hovenbitzer A, Steglich W (1986) Suillin, ein charakteristischer Inhaltsstoff von Röhrlingen der Gattung Suillus (Boletales) Z. Naturforsch 41b:645–648
Besl H, Bresinsky A (1997) Chemosystematics of Suillaceae and Gomphidiaceae (suborder Suillineae). Pl Syst Evol 206:223–242
Tringali C, Piattelli M, Geraci C, Nicolosi G (1989) Antimicrobial tetraprenylphenols from Suillus granulatus. J Nat Prod 52:941–947
Hayashi T, Kanetoshi A, Ikura M, Shirahama H (1989) Bolegrevilol, a new lipid peroxidation inhibitor from the edible mushroom Suillus grevillei. Chem Pharm Bull 37:1424–1425
Kukovinets OS, Zainullin RA, Kislitsyn MI (2006) Natural arylterpenes and their biological activity. Chem Nat Comp 42:1–15
Hirata Y, Nakanishi K (1950) Grifolin, an antibiotic from a Basidiomycete. J Biol Chem 184:135
Vrkoč I, Buděšinský M, Dolejš L (1977) Phenolic meroterpenoids from the Basidiomycete Albatrellus ovinus. Phytochemistry 16:1409–1411
Besl H, Hoefle G, Jendrny B, Jägers E, Steglich W (1977) Pilzpigmente, XXXI. Farnesylphenole aus Albatrellus–Arten (Basidiomycetes). Chem Ber 110:3770–3776
Zechlin L, Wolf M, Steglich W, Anke T (1981) Cristatsäure, ein modifiziertes Farnesylphenol aus Fruchtkörpern von Albatrellus cristatus. Liebigs Ann Chem 12:2099–2105
Hashimoto T, Quang DN, Nukada M, Asakawa Y (2005) Isolation, synthesis and biological activity of grifolic acid derivatives from inedible mushroom Albatrellus dispansus. Heterocycles 65:2431–2439
Nukata M, Hashimoto T, Yamamoto I, Iwasaki N, Tanaka M, Asakawa Y (2002) Neogrifolin derivatives possessing anti-oxidative activity from the mushroom Albatrellus ovinus. Phytochemistry 59:731–737
Misasa H, Matsui Y, Uehara H, Tanaka H, Ishihara M, Shibata H (1992) Tyrosinase inhibitors from Albatrellus confluens. Biosci Biotechnol Biochem 56:1660
Yang XL, Qin C, Wang F, Dong ZJ, Liu JK (2008) A new meroterpenoid pigment from the Basidiomycete Albatrellus confluens. Chem Biodivers 5:484–489
Hellwig V, Nopper R, Mauler F, Freitag J, Ji-Kai L, Zhi-Hui D, Stadler M (2002) Activities of prenylphenol derivatives from fruitbodies of Albatrellus ssp. on the human and rat vanilloid receptor 1 (VR1) and characterisation of the novel natural product, confluentin. Arch Pharm Pharm Med Chem 2:119–126
Lang M, Mühlbauer A, Gräf C, Beyer J, Lang-Fugmann S, Polborn K, Steglich W (2008) Studies on the structure and biosynthesis of tridentoquinone and related meroterpenoids from the mushroom Suillus tridentinus (Boletales). Eur J Org Chem 2008:816–825
Schwarz H, Pasupathy V, Steglich W (1976) Pilzpigmente. XXV. Massenspektrometrische Untersuchung polyisoprenoider Ansabenzochinone. Org Mass Spectrom 11:472–478
Fabre N, Rustan I, de Hoffmann E, Quetin-Leclercq J (2001) Determination of flavone, flavonol, and flavanone aglycones by negative ion liquid chromatography electrospray ion trap mass spectrometry. J Am Soc Mass Spectrom 12:707–715
Lübken T, Arnold N, Wessjohann LA, Böttcher C, Schmidt J (2006) Analysis of fungal cyclopentenone derivatives from Hygrophorus spp. by liquid chromatography/electrospray-tandem mass spectrometry. J Mass Spectrom 41:361–371
Acknowledgements
The authors are indebted to Prof. Dr. Dr. h.c. Wolfgang Steglich and co-workers (Ludwig-Maximilians-University, Munich) for kindly providing the compounds investigated. We thank Petra Majovsky for technical assistance. Ramona Heinke is gratefully acknowledged to the Studienstiftung des Deutschen Volkes for a grant.
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Heinke, R., Arnold, N., Wessjohann, L. et al. Negative ion tandem mass spectrometry of prenylated fungal metabolites and their derivatives. Anal Bioanal Chem 405, 177–189 (2013). https://doi.org/10.1007/s00216-012-6498-1
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DOI: https://doi.org/10.1007/s00216-012-6498-1