Abstract
The present study evaluates the use of an individual-specific transgenerational barium dual-isotope procedure and its application to salmon specimens from the Sella River (Asturias, Spain). For such a purpose, the use of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in combination with multiple linear regression for the determination of the isotopic mark in the otoliths of the specimens is presented. In this sense, a solution in which two barium-enriched isotopes (137Ba and 135Ba) were mixed at a molar ratio of ca. 1:3 (N Ba137/N Ba135) was administered to eight returning females caught during the spawning period. After injection, these females, as well as their offspring, were reared in a governmental hatchery located in the council of Cangas de Onís (Asturias, Spain). For comparison purposes, as well as for a time-monitoring control, egg and larva data obtained by solution analysis ICP-MS are also given. Otoliths (9-month-old juveniles) of marked offspring were analysed by LA-ICP-MS demonstrating a 100 % marking efficacy of this methodology. The capabilities of the molar fraction approach for 2D imaging of fish otoliths are also addressed.
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Acknowledgments
The authors would like to thank Jerónimo de la Hoz and the Dirección General de Recursos Naturales y Protección Ambiental (Gobierno del Principado de Asturias) for their collaboration and care of the marked salmons. Dr. America Garcia-Valiente is acknowledged for the extraction of fish otoliths and Emilio J. Ariño and Joaquín Vázquez for their help with the otolith sample preparation. Financial support through the project MICINN CTQ2009-12814, as well as the provision of FEDER funds for the purchase of the multi-collector ICP-MS instrument, is gratefully acknowledged.
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Published in the topical collection Isotope Ratio Measurements: New Developments and Applications with guest editors Klaus G. Heumann and Torsten C. Schmidt.
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Huelga-Suarez, G., Fernández, B., Moldovan, M. et al. Detection of transgenerational barium dual-isotope marks in salmon otoliths by means of LA-ICP-MS. Anal Bioanal Chem 405, 2901–2909 (2013). https://doi.org/10.1007/s00216-012-6452-2
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DOI: https://doi.org/10.1007/s00216-012-6452-2