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A multiresidual method based on ion-exchange chromatography with conductivity detection for the determination of biogenic amines in food and beverages

Abstract

In the present work a sensitive and accurate method by ion chromatography and conductimetric detection has been developed for the determination of biogenic amines in food samples at microgram per kilogram levels. The optimized extraction procedure of trimethylamine, triethylamine, putrescine, cadaverine, histamine, agmatine, spermidine, and spermine from real samples, as well as the separation conditions based on a multilinear gradient elution with methanesulfonic acid and the use of a weak ionic exchange column, have provided excellent results in terms of resolution and separation efficiency. Extended calibration curves (up to 200 mg/kg, r > 0.9995) were obtained for all the analyzed compounds. The method gave detection limits in the range 23–65 μg/kg and quantification limits in spiked blank real samples in the range 65–198 μg/kg. Recovery values ranged from 82 to 103 %, and for all amines, a good repeatability was obtained with precision levels in the range 0.03–0.32 % (n = 4). The feasibility and potential of the method were tested by the analysis of real samples, such as tinned tuna fish, anchovies, cheese, wine, olives, and salami.

IEC‐CD multiresidual method for accurate determinations of biogenic amines in foodstuffs

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References

  1. European Commission (2005) European Commission regulation (EC) no. 2073/2005, Off. J. Eur. Union L338 1-26

  2. Balamatsia CC, Paleologos EK, Kontominas MG, Savvaidis IN (2006) Correlation between microbial flora, sensory changes and biogenic amines formation in fresh chicken meat stored aerobically or under modified atmosphere packaging at 4 °C: possible role of biogenic amines as spoilage indicators. Anton Leeuw 89:9–17

    Article  CAS  Google Scholar 

  3. Chan ST, Yao MWY, Wong YC, Wong T, Mok CS, Sin DWM (2006) Evaluation of chemical indicators for monitoring freshness of food and determination of volatile amines in fish by headspace solid-phase microextraction and gas chromatography–mass spectrometry. Eur Food Res Technol 224:67–74

    Article  CAS  Google Scholar 

  4. Galgano F, Favati F, Bonadio M, Lorusso V, Romano P (2009) Role of biogenic amines as index of freshness in beef meat packed with different biopolymeric materials. Food Res Int 42:1147–1152

    Article  CAS  Google Scholar 

  5. Tasić T, Ikonić P, Mandić A, Jokanović M, Tomović V, Savatić S, Petrović L (2012) Biogenic amines content in traditional dry fermented sausage Petrovská klobása as possible indicator of good manufacturing practice. Food Control 23:107–112

    Article  Google Scholar 

  6. Krizek M, Pavlicek T, Vacha F (2002) Formation of selected biogenic amines in carp meat. J Sci Food Agr 82:1088–1093

    Article  CAS  Google Scholar 

  7. Silva CMG, GloriaMB A (2002) Bioactive amines in chicken breast and thigh after slaughter and during storage at 4 +/-1 °C and in chicken-based meat products. Food Chem 78:241–248

    Article  CAS  Google Scholar 

  8. Loret S, Deloyer P, Dandrifosse G (2005) Levels of biogenic amines as a measure of the quality of the beer fermentation process: data from Belgian samples. Food Chem 89:519–525

    Article  CAS  Google Scholar 

  9. Adhoum N, Monser L, Sadok S, El-Abed A, Greenway GM, Uglow RF (2003) Flow injection potentiometric detection of trimethylamine in seafood using tungsten oxide electrode. Anal Chim Acta 478:53–58

    Article  CAS  Google Scholar 

  10. Vinci G, Antonelli ML (2002) Biogenic amines: quality index of freshness in red and white meat. Food Control 13:519–524

    Article  CAS  Google Scholar 

  11. Halász A, Baráth A, Simon-Sarkadi L, Holzapfel W (1994) Biogenic amines and their production by microorganisms in food. Trends Food Sci Tech 5:42–49

    Article  Google Scholar 

  12. Kalač P, Krausová P (2005) A review of dietary polyamines: formation, implications for growth and health and occurrence in foods. Food Chem 90:219–230

    Article  Google Scholar 

  13. Önal A (2007) A review: current analytical methods for the determination of biogenic amines in foods. Food Chem 103:1475–1486

    Article  Google Scholar 

  14. Pena-Gallego A, Hernández-Orte P, Cacho J, Ferreira V (2009) Biogenic amine determination in wines using solid-phase extraction: a comparative study. J Chromatogr A 1216:3398–3401

    Article  CAS  Google Scholar 

  15. Triki M, Jiménez-Colmenero F, Herrero AM, Ruiz-Capillas C (2012) Optimisation of a chromatographic procedure for determining biogenic amine concentrations in meat and meat products employing a cation-exchange column with a post-column system. Food Chem 130:1066–1073

    Article  CAS  Google Scholar 

  16. Calbiani F, Careri M, Elviri L, Mangia A, Pistara L, Zagnoni I (2005) Rapid assay for analyzing biogenic amines in cheese: matrix solid-phase dispersion followed by liquid chromatography-electrospray-tandem mass spectrometry. J Agr Food Chem 53(10):3779–3783

    Article  CAS  Google Scholar 

  17. Charles AL (1996) Recent advances in ion chromatography: a perspective. J Chromatogr A 739:3–13

    Article  Google Scholar 

  18. Johnson DC, LaCourse WR (1990) LC with pulsed ECD at gold and platinum. Anal Chem 62:589A–597A

    CAS  Google Scholar 

  19. Draisci R, Cavalli S, Lucentini L, Stacchini A (1993) Ion exchange separation and pulsed amperometric detection for determination of biogenic amines in fish products. Chromatographia 35(9/12):584–590

    Article  CAS  Google Scholar 

  20. Johnson DC, Hoekstra JC (1998) Comparison of potential-time waveforms for the detection of biogenic amines in complex mixtures following their separation by liquid chromatography. Anal Chem 70:83–88

    Article  Google Scholar 

  21. De Borba BM, Rohrer JS (2007) Determination of biogenic amines in alcoholic beverages by ion chromatography with suppressed conductivity detection and integrated pulsed amperometric detection. J Chromatogr A 1155:22–30

    Article  Google Scholar 

  22. Rey M, Pohl C (2003) Novel cation-exchange column for the separation of hydrophobic and/or polyvalent amines. J Chromatogr A 997:199–206

    Article  CAS  Google Scholar 

  23. Cinquina AL, Calì A, Longo F, De Santis L, Severoni A, Abballe F (2004) Determination of biogenic amines in fish tissues by ion-exchange chromatography with conductivity detection. J Chomatogr A 1032:73–77

    Article  CAS  Google Scholar 

  24. Casella IG, Palladino GA, Contursi M (2008) Determination of aliphatic amines by cationexchange chromatography with suppressed conductivity detection after solid phase extraction. J Sep Sci 31:3718–3726

    Article  CAS  Google Scholar 

  25. Favaro G, Pastore P, Saccani G, Cavalli S (2007) Determination of biogenic amines in fresh and processed meat by ion chromatography and integrated pulsed amperometric detection on Au electrode. Food Chem 105:1652–1658

    Article  CAS  Google Scholar 

  26. Saccani G, Tanzi E, Pastore P, Cavalli S, Rey M (2005) Determination of biogenic amines in fresh and processed meat by suppressed ion chromatography-mass spectrometry using a cation-exchange column. J Chromatogr A 1082:43–50

    Article  CAS  Google Scholar 

  27. Draisci R, Giannetti L, Boria P, Lucentini L, Palleschi L, Cavalli S (1998) Improved ion chromatography-integrated pulsed amperometric detection method for the evaluation of biogenic-amines in food of vegetable or animal origin and in fermented foods. J Chromatogr A 798(1–2):109–116

    CAS  Google Scholar 

  28. Arlorio M, Coïsson JD, Martelli A (1999) Extraction methods for biogenic amines in wine and beer. Ita J Food Sci 11(4):355–360

    CAS  Google Scholar 

  29. European Commission (2002) European Commission decision 2002/657/EC, Off. J. Eur. Union L221, 8

  30. European Commission (2004) European Commission regulation (EC) no. 882/2004 of 29 April 2004 Off. J. European Union, L165, pp. 1–141

  31. Self RL, Wu WH, Marks HS (2011) Simultaneous quantification of eight biogenic amine compounds in tuna by matrix solid-phase dispersion followed by HPLC-orbitrap mass spectrometry. J Agric Food Chem 59(11):5906–5913

    Article  CAS  Google Scholar 

  32. Sagratini G, Fernández-Franzón M, De Berardinis F, Font G, Vittori S, Mañes (2012) Simultaneous determination of eight underivatised biogenic amines in fish by solid phase extraction and liquid chromatography–tandem mass spectrometry. J Food Chem 132:537–543

    Article  CAS  Google Scholar 

  33. Ndagijimana M, Belletti N, Gardini F, Vernocchi P, Lanciotti R, Patrignani F (2012) Biogenic amines and ethyl carbamate in primitivo wine: survey of their concentrations in commercial products and relationship with the use of malolactic starter. J Food Protect 75(3):591–596

    Article  Google Scholar 

  34. Yamanaka H, Shiomi K, Kikuchi T (1987) Agmatine as a potential index for freshness of common squid (Todarodes pacificus). J Food Sci 52:936–938

    Article  CAS  Google Scholar 

  35. Kawabata T, Ohshima H, Ino M (1978) Occurrence of methylguanidine and agmatine in foods. IARC Sci Publ 19:415–423

    CAS  Google Scholar 

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Acknowledgments

Ministero della Salute (Rome, Italy) is thanked for providing financial support.

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Correspondence to Carmen Palermo.

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Published in the special issue Analytical Science in Italy with guest editor Aldo Roda.

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Palermo, C., Muscarella, M., Nardiello, D. et al. A multiresidual method based on ion-exchange chromatography with conductivity detection for the determination of biogenic amines in food and beverages. Anal Bioanal Chem 405, 1015–1023 (2013). https://doi.org/10.1007/s00216-012-6439-z

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  • DOI: https://doi.org/10.1007/s00216-012-6439-z

Keywords

  • Ion chromatography
  • Conductivity detection
  • Biogenic amines
  • Linear gradient elution
  • Cation suppression
  • Food