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A multiresidual method based on ion-exchange chromatography with conductivity detection for the determination of biogenic amines in food and beverages

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Abstract

In the present work a sensitive and accurate method by ion chromatography and conductimetric detection has been developed for the determination of biogenic amines in food samples at microgram per kilogram levels. The optimized extraction procedure of trimethylamine, triethylamine, putrescine, cadaverine, histamine, agmatine, spermidine, and spermine from real samples, as well as the separation conditions based on a multilinear gradient elution with methanesulfonic acid and the use of a weak ionic exchange column, have provided excellent results in terms of resolution and separation efficiency. Extended calibration curves (up to 200 mg/kg, r > 0.9995) were obtained for all the analyzed compounds. The method gave detection limits in the range 23–65 μg/kg and quantification limits in spiked blank real samples in the range 65–198 μg/kg. Recovery values ranged from 82 to 103 %, and for all amines, a good repeatability was obtained with precision levels in the range 0.03–0.32 % (n = 4). The feasibility and potential of the method were tested by the analysis of real samples, such as tinned tuna fish, anchovies, cheese, wine, olives, and salami.

IEC‐CD multiresidual method for accurate determinations of biogenic amines in foodstuffs

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Acknowledgments

Ministero della Salute (Rome, Italy) is thanked for providing financial support.

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Authors and Affiliations

  1. Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università degli studi di Foggia, via Napoli, 25, 71122, Foggia, Italy

    Carmen Palermo, Donatella Nardiello & Diego Centonze

  2. Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Università degli studi di Foggia, Via Manfredonia 20, 71122, Foggia, Italy

    Marilena Muscarella & Marco Iammarino

Authors
  1. Carmen Palermo
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  2. Marilena Muscarella
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  3. Donatella Nardiello
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  4. Marco Iammarino
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  5. Diego Centonze
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Correspondence to Carmen Palermo.

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Published in the special issue Analytical Science in Italy with guest editor Aldo Roda.

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Palermo, C., Muscarella, M., Nardiello, D. et al. A multiresidual method based on ion-exchange chromatography with conductivity detection for the determination of biogenic amines in food and beverages. Anal Bioanal Chem 405, 1015–1023 (2013). https://doi.org/10.1007/s00216-012-6439-z

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  • DOI: https://doi.org/10.1007/s00216-012-6439-z

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