Skip to main content
SpringerLink
Log in

Biocatalytic reactors based on ribonuclease A immobilized on macroporous monolithic supports

Analytical and Bioanalytical Chemistry volume 405, pages 2195–2206 (2013)Cite this article

Abstract

Immobilized enzyme reactors (IMERs) produced by the covalent attachment of ribonuclease A to macroporous methacrylate-based monolithic supports using different experimental approaches are discussed and compared. Enzyme immobilization was carried out by direct covalent binding, as well as through attachment via a polymer spacer. The kinetic properties of an IMER operating in either recirculation mode or zonal elution mode were studied. Additionally, the effect of flow rate on the bioconversion efficiency of each IMER sample was examined.

Enzyme immobilization via aldehyde-bearing macromolecular spacer on the surface of epoxy-containing monoliths

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Mancini F, Naldi M, Cavrini V, Andrisano V (2007) J Chromatogr A 1175:217–226

    Article  CAS  Google Scholar 

  2. Sheldon RA (2007) Adv Synth Catal 349:1289–1307

    Article  CAS  Google Scholar 

  3. Battistel E, Bianchi D, Rialdi C (1991) Pure Appl Chem 63:1483–1490

    Article  CAS  Google Scholar 

  4. Josić D, Buchacher A (2001) J Biochem Biophys Methods 49:153–174

    Article  Google Scholar 

  5. Vlakh EG, Tennikova TB (2009) J Chromatogr A 1216:2637–2650

    Article  CAS  Google Scholar 

  6. Vidič J, Podgornik A, Jančar J, Frankovič V, Kosir B, Lendero N, Cuček K, Krajnc M, Štrancar A (2007) J Chromatogr A 1144:63–71

    Article  Google Scholar 

  7. Krenkova J, Svec F (2009) J Sep Sci 32:706–718

    CAS  Google Scholar 

  8. Ma J, Zhang L, Liang Z, Zhang W, Zhang Y (2007) J Sep Sci 30:3050–3059

    Article  CAS  Google Scholar 

  9. Bartolini M, Cavrini V, Andrisano V (2004) J Chromatogr A 1031:27–34

    Article  CAS  Google Scholar 

  10. Bartolini M, Greig NH, Yu QS, Andrizano V (2009) J Chromatogr A 1216:2730–2738

    Article  CAS  Google Scholar 

  11. Kawakami K, Oda Y, Takahashi R (2011) Biotechnol Biofuels 4:42

    Article  CAS  Google Scholar 

  12. Urban J, Svec F, Frechet JMJ (2012) Biotechnol Bioeng 109:371–380

    Article  CAS  Google Scholar 

  13. Delattre C, Michaud P, Vijayalakshmi MA (2008) J Chromatogr B 861:203–208

    Article  CAS  Google Scholar 

  14. Ozoner SK, Keskinler B, Erhan E (2011) Mat Sci Eng C 31:663–668

    Article  CAS  Google Scholar 

  15. Benčina M, Benčina K, Štrancar A, Podgornik A (2005) J Chromatogr A 1065:83–91

    Article  Google Scholar 

  16. Ponomareva EA, Kartuzova VE, Vlakh EG, Tennikova TB (2010) J Chromatogr B 878:567–574

    Article  CAS  Google Scholar 

  17. Raines RT (1998) Chem Rev 98:1045–1065

    Article  CAS  Google Scholar 

  18. Benčina M, Babic J, Podgornik A (2007) J Chromatogr A 1144:135–142

    Article  Google Scholar 

  19. Korzhikov VA, Diederichs S, Nasarova OV, Vlakh EG, Kasper C, Panarin EF, Tennikova TB (2008) J Appl Polym Sci 108:2386–2397

    Article  CAS  Google Scholar 

  20. Rodrigues A, Mata A, Zabka M, Pais L (2003) Chapter 15. In: Svec F, Tennikova TB, Deyl Z (eds) Monolithic materials: preparation, properties and applications. Elsevier, Amsterdam, pp 325–350

  21. Tennikov MB, Gazdina NV, Tennikova TB, Svec F (1998) J Chromatogr A 798:55–64

    Article  CAS  Google Scholar 

  22. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193:265–275

    CAS  Google Scholar 

  23. Benčina M, Benčina K, Podgornik A, Štrancar A (2008) Methods Mol Biol 421:257–274

    Google Scholar 

  24. Stryer L (1996) Biochemistry. W.H. Freeman and Company, New York

  25. Lim YP, Josic D, Callanan H, Brown J, Hixson DC (2005) J Chromatogr A 1065:39–43

    Article  CAS  Google Scholar 

  26. Křenkova J, Bilkova Z, Foret F (2005) J Sep Sci 28:1675–1684

    Article  Google Scholar 

  27. Platonova GA, Vlakh EG, Ivanova ND, Tennikova TB (2009) Russ J Appl Chem 82:2182–2186

    Article  CAS  Google Scholar 

  28. Rogers A, Gibon Y (2009) Chapter 4. In: Schwender J (ed) Plant metabolic networks. Springer, Berlin, pp 71–103

  29. Bailey JE, Ollis DF (1986) Biochemical engineering fundamentals. McGraw-Hill, New York

    Google Scholar 

  30. Hage DS (2005) Chapter 35. In: Cazes J (ed) Encyclopedia of chromatography. CRC, Boca Raton, pp 184–190

  31. Temporini C, Perani E, Mancini F, Bartolini M, Calleri E, Lubda D, Felix G, Andrisano V, Massolini G (2006) J Chromatogr A 1120:121–131

    Article  CAS  Google Scholar 

  32. Calleri E, Temporini C, Gasparrini F, Simone P, Villani C, Ciogli A, Massolini G (2011) J Chromatogr A 1218:8937–8945

    Article  CAS  Google Scholar 

  33. Nicoli R, Gaud N, Stella C, Rudaz S, Veuthey JL (2008) J Pharm Biomed Anal 48:398–407

    Article  CAS  Google Scholar 

  34. Petro M, Svec F, Fréchet JMJ (1996) Biotech Bioeng 49:355–363

    Article  CAS  Google Scholar 

  35. Duan J, Liang Z, Yang C, Zhang J, Zhang L, Zhang W, Zhang Y (2006) Proteomics 6:412–419

    Article  CAS  Google Scholar 

  36. Xie S, Svec F, Frechet JMJ (1999) Biotech Bioeng 62:30–35

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Russian Foundation of Basic Research (grant RFBR #11-03-00829-a) and the Russian Federal Program “Research and scientific-pedagogical personnel of innovative Russia in 2009–2013” (contract #14.740.11.0382). The authors are very grateful to BIA Separations Company (Ljubljana, Slovenia) for their kind donation of the CIM disks.

Author information

Authors and Affiliations

  1. Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004, St. Petersburg, Russia

    E. A. Ponomareva, M. V. Volokitina, E. G. Vlakh & T. B. Tennikova

  2. Faculty of Chemical and Biochemical Technology, Saint Petersburg State Institute of Technology (Technical University), Moskovsky pr. 26, 190013, St. Petersburg, Russia

    M. V. Volokitina & D. O. Vinokhodov

  3. Faculty of Chemistry, Saint Petersburg State University, Universitesky pr. 26, Petrodvorez, 198584, St. Petersburg, Russia

    E. G. Vlakh & T. B. Tennikova

Authors
  1. E. A. Ponomareva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. V. Volokitina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. O. Vinokhodov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. G. Vlakh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. B. Tennikova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. B. Tennikova.

Additional information

Published in the topical collection Monolithic Columns in Liquid Phase Separations with guest editor Luis A. Colon.

Electronic supplementary material

A'dditional figures (as noted in the text) are available on the journal website.

ESM 1

(PDF 102 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ponomareva, E.A., Volokitina, M.V., Vinokhodov, D.O. et al. Biocatalytic reactors based on ribonuclease A immobilized on macroporous monolithic supports. Anal Bioanal Chem 405, 2195–2206 (2013). https://doi.org/10.1007/s00216-012-6391-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-012-6391-y

Keywords

search

Navigation