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Analytical and Bioanalytical Chemistry

, Volume 405, Issue 7, pp 2195–2206 | Cite as

Biocatalytic reactors based on ribonuclease A immobilized on macroporous monolithic supports

  • E. A. Ponomareva
  • M. V. Volokitina
  • D. O. Vinokhodov
  • E. G. Vlakh
  • T. B. TennikovaEmail author
Original Paper

Abstract

Immobilized enzyme reactors (IMERs) produced by the covalent attachment of ribonuclease A to macroporous methacrylate-based monolithic supports using different experimental approaches are discussed and compared. Enzyme immobilization was carried out by direct covalent binding, as well as through attachment via a polymer spacer. The kinetic properties of an IMER operating in either recirculation mode or zonal elution mode were studied. Additionally, the effect of flow rate on the bioconversion efficiency of each IMER sample was examined.

Figure

Enzyme immobilization via aldehyde-bearing macromolecular spacer on the surface of epoxy-containing monoliths

Keywords

Polymer monoliths Enzyme immobilization Flow-through immobilized enzyme reactors Polymer spacer 

Notes

Acknowledgments

This work was supported by the Russian Foundation of Basic Research (grant RFBR #11-03-00829-a) and the Russian Federal Program “Research and scientific-pedagogical personnel of innovative Russia in 2009–2013” (contract #14.740.11.0382). The authors are very grateful to BIA Separations Company (Ljubljana, Slovenia) for their kind donation of the CIM disks.

Supplementary material

216_2012_6391_MOESM1_ESM.pdf (103 kb)
ESM 1 (PDF 102 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • E. A. Ponomareva
    • 1
  • M. V. Volokitina
    • 1
    • 2
  • D. O. Vinokhodov
    • 2
  • E. G. Vlakh
    • 1
    • 3
  • T. B. Tennikova
    • 1
    • 3
    Email author
  1. 1.Institute of Macromolecular CompoundsRussian Academy of SciencesSt. PetersburgRussia
  2. 2.Faculty of Chemical and Biochemical TechnologySaint Petersburg State Institute of Technology (Technical University)St. PetersburgRussia
  3. 3.Faculty of ChemistrySaint Petersburg State UniversitySt. PetersburgRussia

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