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Differential immuno-kinetic assays of allergen-specific binding for peanut allergy serum analysis

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Abstract

A label-free nanoparticle array platform has been used to detect total peanut allergen-specific binding from whole serum of patients suffering from peanut allergy. The serum from 10 patients was screened against a four-allergen panel of cat and dog dander, dust mite and peanut allergen protein Ara h1. The IgE and IgG contributions to the total specific-binding protein load to Ara h1 were identified using two secondary IgG- and IgE-specific antibodies and were found to contribute less than 50 % of the total specific protein load. The total mass of IgE, IgE and the unresolved specific-binding protein ΔsBP for Ara h1 provides a new serum profile for high-RAST-grade patients 5 and 6 with the IgG/IgE ratio of 4 ± 2 and ΔsBP/IgE ratio of 17 ± 11, neither of which is protective for the small patient cohort.

Binding of the specefic IgE/IgG and unresolved material X to the sensor surface functionalized with allergen

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Acknowledgments

The research was funded by the BBSRC and the EPSRC.

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Authors and Affiliations

  1. College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4QD, UK

    Rouslan V. Olkhov & Andrew M. Shaw

  2. Department of Immunology, Derriford Hospital, Derriford Road, Plymouth, PL6 8DH, UK

    Edward R. Kaminski

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  1. Rouslan V. Olkhov
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  2. Edward R. Kaminski
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  3. Andrew M. Shaw
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Correspondence to Andrew M. Shaw.

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Olkhov, R.V., Kaminski, E.R. & Shaw, A.M. Differential immuno-kinetic assays of allergen-specific binding for peanut allergy serum analysis. Anal Bioanal Chem 404, 2241–2247 (2012). https://doi.org/10.1007/s00216-012-6390-z

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  • DOI: https://doi.org/10.1007/s00216-012-6390-z

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