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Atomic force microscopy of model lipid membranes

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Abstract

Supported lipid bilayers (SLBs) are biomimetic model systems that are now widely used to address the biophysical and biochemical properties of biological membranes. Two main methods are usually employed to form SLBs: the transfer of two successive monolayers by Langmuir–Blodgett or Langmuir–Schaefer techniques, and the fusion of preformed lipid vesicles. The transfer of lipid films on flat solid substrates offers the possibility to apply a wide range of surface analytical techniques that are very sensitive. Among them, atomic force microscopy (AFM) has opened new opportunities for determining the nanoscale organization of SLBs under physiological conditions. In this review, we first focus on the different protocols generally employed to prepare SLBs. Then, we describe AFM studies on the nanoscale lateral organization and mechanical properties of SLBs. Lastly, we survey recent developments in the AFM monitoring of bilayer alteration, remodeling, or digestion, by incubation with exogenous agents such as drugs, proteins, peptides, and nanoparticles.

The experimental atomic force microscopy (AFM) setup used to examine supported lipid bilayers (SLBs) under physiological conditions.

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Acknowledgments

The authors thank Yves Dufrene for the valuable suggestions and discussions. Financial support of the Centre National de la Recherche Scientifique (CNRS), of the French Research Ministry, of the Université de Technologie de Compiègne (UTC, Plan de Pluriformation “Assemblages Biomimétiques - PPF BIOMIM”), and of the Région Picardie (“Ce projet est cofinancé par l’Union Européenne. L’Europe s’engage en Picardie avec le Fonds Européen de Développement Régional”) is gratefully acknowledged.

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  1. UMR 6022 Laboratoire de Génie Enzymatique et Cellulaire, Université de Technologie de Compiègne – CNRS, BP 20529, 60205, Compiègne Cedex, France

    Sandrine Morandat, Slim Azouzi, Estelle Beauvais & Amira Mastouri

  2. UMR 7338 Laboratoire de Biomécanique et Bioingénierie, Université de Technologie de Compiègne – CNRS, BP 20529, 60205, Compiègne Cedex, France

    Slim Azouzi, Estelle Beauvais, Amira Mastouri & Karim El Kirat

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  1. Sandrine Morandat
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  2. Slim Azouzi
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Correspondence to Karim El Kirat.

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Published in the topical collection Characterization of Thin Films and Membranes with guest editors Daniel Mandler and Pankaj Vadgama.

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Morandat, S., Azouzi, S., Beauvais, E. et al. Atomic force microscopy of model lipid membranes. Anal Bioanal Chem 405, 1445–1461 (2013). https://doi.org/10.1007/s00216-012-6383-y

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