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Chemoselective reduction-based fluorescence probe for detection of hydrogen sulfide in living cells

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Abstract

A selective and sensitive fluorescence probe for hydrogen sulfide (H2S) detection was synthesized and evaluated in PBS buffer and fetal bovine serum. The effect of pH on the probe was also studied. In addition, visualization of H2S in Hela cells was achieved using confocal laser scanning fluorescence microscopy.

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Acknowledgments

We thank Dr. Jianchun Zhou for assistance of all imaging experiments and Prof. Vasudevan Ramesh (University of Manchester, UK) for reading this manuscript. This work was supported by the National Natural Science Foundation of China (grant no. 21072015), Program for New Century Excellent Talents in University (NCET-10-0203), and Innovation Team of Ministry of Education (no. BMU20110263).

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Authors and Affiliations

  1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38, Xueyuan Rd., Beijing, 100191, China

    Bifeng Chen, Cong Lv & Xinjing Tang

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  1. Bifeng Chen
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  2. Cong Lv
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  3. Xinjing Tang
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Correspondence to Xinjing Tang.

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Chen, B., Lv, C. & Tang, X. Chemoselective reduction-based fluorescence probe for detection of hydrogen sulfide in living cells. Anal Bioanal Chem 404, 1919–1923 (2012). https://doi.org/10.1007/s00216-012-6292-0

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  • DOI: https://doi.org/10.1007/s00216-012-6292-0

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