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Pulsed supersonic beams with nucleobases

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Abstract

The dissolution of the primary nucleobases in supercritical fluids has been investigated using pulsed molecular beam mass spectrometry. Due to the low critical temperatures of ethylene and carbon dioxide, their adiabatic jet expansion permits transferring thermally sensitive solutes into the gas phase. This feature is particularly attractive for pharmaceutical and biomedical applications. In this study, adenine, guanine, cytosine, thymine, and uracil have been dissolved in supercritical ethylene with a few percent of ethanol as cosolvent. At source temperatures of 313 K, these solutions have been expanded from supercritical pressures into high vacuum using a customized pulsed nozzle. A mass spectrometer was used to monitor the relative amounts of solute, solvent, and cosolvent in the supersonic beam. The results suggest a paramount influence of the cosolvent.

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References

  1. Scoles G (ed) (1988) Atomic and molecular beam methods. Oxford University Press, New York

    Google Scholar 

  2. Pauly H (2000) Atom, molecule, and cluster beams. Springer, New York

    Google Scholar 

  3. Campargue R (ed) (2001) Atomic and molecular beams. Springer, New York

    Google Scholar 

  4. Randall LG, Wahrhaftig AL (1978) Anal Chem 50:1703

    Article  CAS  Google Scholar 

  5. Shinozaki H, Oguchi T, Suzuki S, Aoki K, Sako T, Morishita S, Tozuka Y, Moribe K, Yamamoto K (2006) Drug Dev Ind Pharm 32:877

    Article  CAS  Google Scholar 

  6. Subramaniam B, Rajewski RA, Snavely K (1997) J Pharm Sci 86:885

    Article  CAS  Google Scholar 

  7. Christen W, Geggier S, Grigorenko S, Rademann K (2004) Rev Sci Instrum 75:5048

    Article  CAS  Google Scholar 

  8. Zhang Q, Wodtke AM (2005) Anal Chem 77:7612

    Article  CAS  Google Scholar 

  9. Christen W, Krause T, Rademann K (2008) Int J Mass Spectrom 277:305

    Google Scholar 

  10. Christen W, Rademann K, Even U (2006) J Chem Phys 125:174307

    Article  Google Scholar 

  11. De Dea S, Miller DR, Continetti RE (2009) J Phys Chem A 113:388

    Article  Google Scholar 

  12. Östblom M, Liedberg B, Demers LM, Mirkin CA (2005) J Phys Chem B 109:15150

    Article  Google Scholar 

  13. Kershner RJ, Bozano LD, Micheel CM, Hung AM, Fornof AR,Cha JN, Rettner CT, Bersani M, Frommer J, Rothemund PWK, Wallraff GM (2009) Nature Nanotech 4:557

    Article  CAS  Google Scholar 

  14. Huang S, Chang S, He J, Zhang P, Liang F, Tuchband M, Li S, Lindsay S (2010) J Phys Chem C 114:20443

    Article  CAS  Google Scholar 

  15. Singh P, Toma FM, Kumar J, Venkatesh V, Raya J, Prato M, Verma S, Bianco A (2011) Chem Eur J 17:6772

    Article  CAS  Google Scholar 

  16. Mozejko P, Sanche L (2003) Radiat Environ Biophys 42:201

    Article  CAS  Google Scholar 

  17. Stein SE (2010) Mass spectra in NIST Chemistry WebBook, NIST standard reference database number 69. In: PJ Linstrom and WG Mallard (eds), National Institute of Standards and Technology, Gaithersburg MD

  18. Dobbs JM, Wong JM, Lahiere RJ, Johnston KP (1987) Ind Eng Chem Res 26:1

    Article  Google Scholar 

  19. Ting SST, Tomasko DL, Foster NR, Macnaughton SJ (1993) Ind Eng Chem Res 32:1471

    Article  CAS  Google Scholar 

  20. Huang Z, Lu WD, Kawi S, Chiew YC (2004) J Chem Eng Data 49:1323

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work has been financially supported by grant CH262/5 from the Deutsche Forschungsgemeinschaft and also from the Max Planck Society through the “International Max Planck Research School: Complex Surfaces in Material Science” at the Fritz Haber Institute Berlin.

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Authors and Affiliations

  1. Institut für Chemie, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489, Berlin, Germany

    Adnan Sarfraz, Klaus Rademann & Wolfgang Christen

Authors
  1. Adnan Sarfraz
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  2. Klaus Rademann
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  3. Wolfgang Christen
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Correspondence to Wolfgang Christen.

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Sarfraz, A., Rademann, K. & Christen, W. Pulsed supersonic beams with nucleobases. Anal Bioanal Chem 404, 2087–2090 (2012). https://doi.org/10.1007/s00216-012-6275-1

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  • DOI: https://doi.org/10.1007/s00216-012-6275-1

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