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High-speed X-ray microscopy by use of high-resolution zone plates and synchrotron radiation

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Abstract

X-ray microscopy based on synchrotron radiation has become a fundamental tool in biology and life sciences to visualize the morphology of a specimen. These studies have particular requirements in terms of radiation damage and the image exposure time, which directly determines the total acquisition speed. To monitor and improve these key parameters, we present a novel X-ray microscopy method using a high-resolution zone plate as the objective and the matching condenser. Numerical simulations based on the scalar wave field theory validate the feasibility of the method and also indicate the performance of X-ray microscopy is optimized most with sub-10-nm-resolution zone plates. The proposed method is compatible with conventional X-ray microscopy techniques, such as computed tomography, and will find wide applications in time-resolved and/or dose-sensitive studies such as living cell imaging.

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Acknowledgments

We acknowledge Wenbing Yun and Augusto Marcelli for valuable suggestions. This work was partly supported by the National Basic Research Program of China (2012CB825800, 2009CB930804), the Knowledge Innovation Program of the Chinese Academy of Sciences (KJCX2-YW-N42), the Key Important Project of the National Natural Science Foundation of China (10734070), the Fundamental Research Funds for the Central Universities (WK2310000008, WK2310000021), and the China Postdoctoral Science Foundation (2011M501064)

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Authors and Affiliations

  1. National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, 230029, China

    Qiyue Hou, Zhili Wang, Kun Gao, Zhiyun Pan, Dajiang Wang, Xin Ge & Ziyu Wu

  2. Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China

    Kai Zhang, Youli Hong, Peiping Zhu & Ziyu Wu

Authors
  1. Qiyue Hou
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  2. Zhili Wang
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  3. Kun Gao
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  4. Zhiyun Pan
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  5. Dajiang Wang
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  6. Xin Ge
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  7. Kai Zhang
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  8. Youli Hong
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  9. Peiping Zhu
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  10. Ziyu Wu
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Corresponding authors

Correspondence to Zhili Wang or Ziyu Wu.

Additional information

Published in the special paper collection Imaging Techniques with Synchrotron Radiation with guest editor Cyril Petibois.

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Hou, Q., Wang, Z., Gao, K. et al. High-speed X-ray microscopy by use of high-resolution zone plates and synchrotron radiation. Anal Bioanal Chem 404, 1327–1330 (2012). https://doi.org/10.1007/s00216-012-6218-x

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  • DOI: https://doi.org/10.1007/s00216-012-6218-x

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