Skip to main content
SpringerLink
Log in

Preparation of a magnetic molecularly imprinted polymer with pseudo template for rapid simultaneous determination of cyromazine and melamine in bio-matrix samples

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

A magnetic molecularly imprinted polymer (M-MIP) for cyromazine and melamine was prepared by simple suspension polymerization using a pseudo template, 2-(4,6-diamino-1,3,5-triazin-2-ylamino)ethanethiol disulfide. The M-MIP was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, and vibrating sample magnetometry. Molecular recognition properties and binding capability to cyromazine and melamine were evaluated by adsorption testing, which showed the M-MIP had better affinity and selectivity than the magnetic non-imprinted polymer (M-NIP) for cyromazine and melamine. A method based on molecularly imprinted solid-phase extraction assisted by magnetic separation was developed for extraction of cyromazine and melamine from bio-matrix samples. Various conditions, for example desorption conditions, amount of M-MIP, extraction time, and sample pH were optimized. High-performance liquid chromatography with UV detection was used to determine cyromazine and melamine after extraction. The proposed method was successfully applied to determination of cyromazine and melamine in egg and milk samples. Recovery of standard spiked cyromazine and melamine from these samples was between 71.86 and 80.57 %, with intraday and interday relative standard deviation ranging from 3.45 to 6.39 % and from 3.95 to 7.84 %, respectively. The results indicate that the pseudo template M-MIP can be used for preconcentration, purification, and analysis of cyromazine and melamine in bio-matrix samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Alexander C, Andersson HS, Andersson LI, Ansell RJ, Kirsch N, Nicholls IA, Mahony JO, Whitcombe MJ (2006) J Mol Recognit 19:106–180

    Article  CAS  Google Scholar 

  2. Kandimalla VB, Ju HX (2004) Anal Bioanal Chem 380:587–605

    Article  CAS  Google Scholar 

  3. Davies MP, Biasi VD, Perrett D (2004) Anal Chim Acta 504:7–14

    Article  CAS  Google Scholar 

  4. Wulff G (2002) Chem Rev 102:1–27

    Article  CAS  Google Scholar 

  5. Tamayo FG, Turiel E, Esteban AM (2007) J Chromatogr A 1152:32–40

    Article  CAS  Google Scholar 

  6. Beltran A, Marce RM, Cormack PAG, Borrull F (2009) J Chromatogr A 1216:2248–2253

    Article  CAS  Google Scholar 

  7. Chen LX, Xu SF, Li JH (2011) Chem Soc Rev 40:2922–2942

    Article  CAS  Google Scholar 

  8. Masque N, Marce RM, Borrull F, Cormack PAG, Sherrington DC (2000) Anal Chem 72:4122–4126

    Article  CAS  Google Scholar 

  9. Sellergren B (1994) Anal Chem 66:1578–1582

    Article  CAS  Google Scholar 

  10. Tsang SC, Caps V, Paraskevas I, Chadwick D, Thompsett D (2004) Angew Chem Int Ed 43:5645–5649

    Article  CAS  Google Scholar 

  11. Zhang Y, Liu RJ, Hu YL, Li GK (2009) Anal Chem 81:967–976

    Article  CAS  Google Scholar 

  12. Hu YL, Li YW, Liu RJ, Tan W, Li GK (2011) Talanta 84:462–470

    Article  CAS  Google Scholar 

  13. Chen LG, Liu J, Zeng QL, Wang H, Yu AM, Zhang HQ, Ding L (2009) J Chromatogr A 1216:3710–3719

    Article  CAS  Google Scholar 

  14. Chen LG, Zhang XP, Sun L, Xu Y, Zeng QL, Wang H, Xu HY, Yu AM, Zhang HQ, Ding L (2009) J Agric Food Chem 57:10073–10080

    Article  CAS  Google Scholar 

  15. Ji YS, Yin JJ, Xu ZG, Zhao CD, Huang HY, Zhang HX, Wang CM (2009) Anal Bioanal Chem 395:1125–1133

    Article  CAS  Google Scholar 

  16. Chen LG, Zhang XP, Xu Y, Du XB, Sun X, Sun L, Wang H, Zhao Q, Yu AM, Zhang HQ, Ding HQ (2010) Anal Chim Acta 662:31–38

    Article  CAS  Google Scholar 

  17. Cook AM, Hutter R (1981) J Agric Food Chem 29:1135–1143

    Article  CAS  Google Scholar 

  18. Julie R, Ingelfinger MD (2008) N Engl J Med 359:2745–2748

    Article  Google Scholar 

  19. Wang H, Dong YH, An Q (2008) Trans ASAE 24:246–249

    Google Scholar 

  20. Dorne JL, Doerge DR, Vandenbroeck M, Fink-Gremmels J, Mennes W, Knutsen HK, Vernazza F, Castle L, Edler L, Benford D (2012) Toxicol Appl Pharmacol. doi:10.1016/j.taap.2012.01.012

  21. Puschner B, Poppenga RH, Lowenstine LJ, Filigenzi MS, Pesavento PA (2007) J Vet Diagn Invest 19:616–624

    Article  Google Scholar 

  22. Brown CA, Jeong KS, Poppenga RH, Puschner B, Miller DM, Ellis AE, Kang KI, Sum S, Cistola AM, Brown SA (2007) J Vet Diagn Invest 19:525–531

    Article  Google Scholar 

  23. Ai KL, Liu YL, Lu LH (2009) J Am Chem Soc 131:9496–9497

    Article  CAS  Google Scholar 

  24. Pietrzyk A, Kutner W, Chitta R, Zandler ME, Souza FD, Sannicolo F, Mussini PR (2009) Anal Chem 81:10061–10070

    Article  CAS  Google Scholar 

  25. Liang RN, Zhang RM, Qin W (2009) Sens Actuators B 141:544–550

    Article  Google Scholar 

  26. Mauer LJ, Chernyshova AA, Hiatt A, Deering A, Davis R (2009) J Agric Food Chem 57:3974–3980

    Article  CAS  Google Scholar 

  27. Zhu L, Gamez G, Chen HW, Chingin K, Zenobi R (2009) Chem Commun 559:559–561

    Article  Google Scholar 

  28. Cabras P, Melon M, Spanedda L (1990) J Chromatogr 505:413–416

    Article  CAS  Google Scholar 

  29. Chou SS, Hwang D, Lee HF (2003) J Food Drug Anal 11:290–295

    CAS  Google Scholar 

  30. Sun HW, Wang LX, Liu N, Qiao FX, Liang SX (2009) Chromatographia 70:1685–1689

    Article  CAS  Google Scholar 

  31. Xia K, Atakins J, Foster C, Armbrust K (2010) J Agric Food Chem 58:5945–5949

    Article  CAS  Google Scholar 

  32. Sancho JV, Ibanez M, Grimalt S, Pozo OJ, Hernandez F (2005) Anal Chim Acta 530:237–243

    Article  CAS  Google Scholar 

  33. Xia JG, Zhou NY, Liu YJ, Chen B, Wu YN, Yao SZ (2010) Food Control 21:912–918

    Article  CAS  Google Scholar 

  34. Zhu XL, Wang SH, Liu O, Xu Q, Xu SX, Chen HL (2009) J Agric Food Chem 57:11075–11080

    Article  CAS  Google Scholar 

  35. Yokley RA, Mayer LC, Rezaaiyan R, Manuli ME, Cheung MW (2000) J Agric Food Chem 48:3352–3358

    Article  CAS  Google Scholar 

  36. Yang HH, Zhou WH, Guo XC, Chen FR, Zhao HQ, Lin LM, Wang XR (2009) Talanta 80:821–825

    Article  CAS  Google Scholar 

  37. He LM, Su YJ, Shen XG, Zheng YQ, Guo HB, Zeng ZL (2009) J Sep Sci 32:3310–3318

    Article  CAS  Google Scholar 

  38. He LM, Su YJ, Zheng YQ, Huang XH, Wu L, Liu YH, Zeng ZL, Chen ZL (2009) J Chromatogr A 1216:6196–6203

    Article  CAS  Google Scholar 

  39. Curcio M, Puoci F, Cirillo G, Iemma F, Spizzirri UG, Picci N (2010) J Agric Food Chem 58:11883–11887

    Article  CAS  Google Scholar 

  40. Wang SS, Li DM, Hua ZD, Zhao MP (2011) Analyst 136:3672–3679

    Article  CAS  Google Scholar 

  41. Ma MM, Paredes A, Bong D (2008) J Am Chem Soc 130:14456–14458

    Article  CAS  Google Scholar 

  42. Wulff G (1995) Angew Chem Int Ed Engl 34:1812–1832

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 20775053 and 20875067).

Author information

Authors and Affiliations

  1. School of Pharmaceutical Science and Technology, Tianjin University, Room A405, Building #24, 92 Weijin Road, Nankai District, Tianjin, 300072, China

    Xianhua Wang, Qiuxue Fang, Shipeng Liu & Lei Chen

Authors
  1. Xianhua Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qiuxue Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shipeng Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lei Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, X., Fang, Q., Liu, S. et al. Preparation of a magnetic molecularly imprinted polymer with pseudo template for rapid simultaneous determination of cyromazine and melamine in bio-matrix samples. Anal Bioanal Chem 404, 1555–1564 (2012). https://doi.org/10.1007/s00216-012-6200-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-012-6200-7

Keywords

Search

Navigation