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Acoustic deposition with NIMS as a high-throughput enzyme activity assay

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Abstract

Mass spectrometry (MS)-based enzyme assay has been shown to be a useful tool for screening enzymatic activities from environmental samples. Recently, reported approaches for high-specificity multiplexed characterization of enzymatic activities allow for providing detailed information on the range of enzymatic products and monitoring multiple enzymatic reactions. However, the throughput has been limited by the slow liquid–liquid handling and manual analysis. This rapid communication demonstrates the integration of acoustic sample deposition with nanostructure initiator mass spectrometry (NIMS) imaging to provide reproducible measurements of multiple enzymatic reactions at a throughput that is tenfold to 100-fold faster than conventional MS-based enzyme assay. It also provides a simple means for the visualization of multiple reactions and reaction pathways.

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Acknowledgment

This work was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy.

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Authors and Affiliations

  1. Nextval, 4186 Sorrento Valley Blvd, Suite G, San Diego, CA, 92121, USA

    Matthew Greving, Michael Nyman & Joseph Cohen

  2. Department of Bioenergy/GTL and Structural Biology, Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94704, USA

    Xiaoliang Cheng, Wolfgang Reindl, Benjamin Bowen, Katherine Louie & Trent Northen

  3. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Joint BioEnergy Institute (JBEI), Berkeley, CA, 94720, USA

    Xiaoliang Cheng, Wolfgang Reindl, Kai Deng, Anup Singh, Blake Simmons, Paul Adams & Trent Northen

  4. Biotechnology and Bioengineering and Biomass Science and Conversion, Technology Departments, Sandia National Laboratories, Livermore, CA, 94551-0969, USA

    Kai Deng, Anup Singh & Blake Simmons

  5. Department of Chemistry, Center for Metabolomics, The Scripps Research Institute, La Jolla, CA, 92037, USA

    Gary Siuzdak

Authors
  1. Matthew Greving
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  2. Xiaoliang Cheng
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  3. Wolfgang Reindl
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  4. Benjamin Bowen
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  5. Kai Deng
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  6. Katherine Louie
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  7. Michael Nyman
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  8. Joseph Cohen
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  9. Anup Singh
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  10. Blake Simmons
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  11. Paul Adams
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  12. Gary Siuzdak
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  13. Trent Northen
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Corresponding author

Correspondence to Trent Northen.

Additional information

M. Greving and X. Cheng contributed equally to this work.

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Greving, M., Cheng, X., Reindl, W. et al. Acoustic deposition with NIMS as a high-throughput enzyme activity assay. Anal Bioanal Chem 403, 707–711 (2012). https://doi.org/10.1007/s00216-012-5908-8

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  • DOI: https://doi.org/10.1007/s00216-012-5908-8

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