Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads


We report here the development of a new assay for the detection of ochratoxin A (OTA) based on the use of its dechlorinated analogue, ochratoxin B (OTB), in a displacement immunoassay. OTB was immobilised on controlled-pore glass beads followed by the binding of anti-OTA antibody, with anti-IgG antibody peroxidase conjugate used as a label. In this manner, an original bio-sensing material was obtained. Upon incubation of this material with OTA, the toxin competes with OTB for the binding sites of the anti-OTA antibodies and releases the antibody-tagged peroxidase complex into the solution. Compared to classic competitive immunoassays, this newly developed displacement immunoassay presents a similar detection limit and assay time. Moreover, the detection, based on the activity of the horseradish peroxidase, is performed for the first time in situ using wine samples.

Steps in the construction of the OTB based bio-sensing glass material (1–5) and the displacement reaction (step 6) upon incubation with ochratoxin A

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The authors gratefully acknowledge the “Agence Universitaire de la Francophonie” (AUF) for funding Vasilica Lates.

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Correspondence to Jean-Louis Marty.

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Lates, V., Yang, C., Popescu, I.C. et al. Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads. Anal Bioanal Chem 402, 2861–2870 (2012). https://doi.org/10.1007/s00216-012-5721-4

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  • Ochratoxin B
  • Displacement immunoassay
  • Controlled-pore glass
  • Ochratoxin A