We report here the development of a new assay for the detection of ochratoxin A (OTA) based on the use of its dechlorinated analogue, ochratoxin B (OTB), in a displacement immunoassay. OTB was immobilised on controlled-pore glass beads followed by the binding of anti-OTA antibody, with anti-IgG antibody peroxidase conjugate used as a label. In this manner, an original bio-sensing material was obtained. Upon incubation of this material with OTA, the toxin competes with OTB for the binding sites of the anti-OTA antibodies and releases the antibody-tagged peroxidase complex into the solution. Compared to classic competitive immunoassays, this newly developed displacement immunoassay presents a similar detection limit and assay time. Moreover, the detection, based on the activity of the horseradish peroxidase, is performed for the first time in situ using wine samples.
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The authors gratefully acknowledge the “Agence Universitaire de la Francophonie” (AUF) for funding Vasilica Lates.
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Lates, V., Yang, C., Popescu, I.C. et al. Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads. Anal Bioanal Chem 402, 2861–2870 (2012). https://doi.org/10.1007/s00216-012-5721-4
- Ochratoxin B
- Displacement immunoassay
- Controlled-pore glass
- Ochratoxin A