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Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

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An Erratum to this article was published on 10 March 2012

Abstract

Fluoroquinolones are one of the most promising and intensively studied drugs of contemporary anti-infective chemotherapy. New fluoroquinolone antibacterials with improved pharmacokinetic properties and a broad spectrum of activity have been developed, opening new windows of opportunity for clinical use. To our knowledge, no comprehensive and critical review of the analytical methods for the determination of these agents, which correspond to the third- and fourth-generation quinolones, has yet been published. This work summarizes for the first time most of the liquid chromatographic methods reported in the literature for the separation and quantification of the new fluoroquinolones in biological matrices and pharmaceutical formulations. A systematic and detailed survey of physicochemical properties, sample preparation procedures, and chromatographic and detection conditions is presented herein. In the course of this review several liquid chromatographic methods are discussed: reversed-phase high-performance liquid chromatography (RP-HPLC), ion-exchange high-performance liquid chromatography (IEX-HPLC), hydrophilic interaction liquid chromatography (HILIC), high-performance thin-layer chromatography (HPTLC) and other chiral chromatographic methods. Their advantages, applicability and limitations are also examined.

Liquid chromatographic methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations.

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Acknowledgments

This work was supported by Fundação para a Ciência e a Tecnologia through the grant SFRH/BD/69378/2010.

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Authors and Affiliations

  1. Pharmacology Department, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal

    Joana Sousa, Ana Fortuna & Amílcar Falcão

  2. CNC – Centre for Neurosciences and Cell Biology, University of Coimbra, 3004-517, Coimbra, Portugal

    Joana Sousa, Gilberto Alves, Ana Fortuna & Amílcar Falcão

  3. CICS-UBI – Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal

    Gilberto Alves

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  1. Joana Sousa
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  2. Gilberto Alves
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  4. Amílcar Falcão
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Correspondence to Amílcar Falcão.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00216-012-5913-y.

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Sousa, J., Alves, G., Fortuna, A. et al. Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review. Anal Bioanal Chem 403, 93–129 (2012). https://doi.org/10.1007/s00216-011-5706-8

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