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Preparation and certification of Hijiki reference material, NMIJ CRM 7405-a, from the edible marine algae hijiki (Hizikia fusiforme)

An Erratum to this article was published on 16 May 2012


A certified reference material, NMIJ CRM 7405-a, for the determination of trace elements and As(V) in algae was developed from the edible marine hijiki (Hizikia fusiforme) and certified by the National Metrology Institute of Japan (NMIJ), the National Institute of Advanced Industrial Science and Technology (AIST). Hijiki was collected from the Pacific coast in the Kanto area of Japan, and was washed, dried, powdered, and homogenized. The hijiki powder was placed in 400 bottles (ca. 20 g each). The concentrations of 18 trace elements and As(V) were determined by two to four independent analytical techniques, including (ID)ICP-(HR)MS, ICP-OES, GFAAS, and HPLC-ICP-MS using calibration solutions prepared from the elemental standard solution of Japan calibration service system (JCSS) and the NMIJ CRM As(V) solution, and whose concentrations are certified and SI traceable. The uncertainties of all the measurements and preparation procedures were evaluated. The values of 18 trace elements and As(V) in the CRM were certified with uncertainty (k = 2).

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  1. 1.

    Yoshinaga J, Shibata Y, Horiguchi T, Morita M (1997) Accredit Qual Assur 2:154–156

    Article  CAS  Google Scholar 

  2. 2.

    Food Standards Agency. Seaweed warning, Wednesday 28 July, (2004)

  3. 3.

    Ministry of Health, Labor and Welfare of Japan, Q&A on arsenic in Hijiki seaweed [in Japanese] (2004)

  4. 4.

    WHO Evaluation of certain food additives and contaminants (1989) 33rd Report of the Joint FAO/WHO Expert Committee on Food Additives; WHO Technical Report Series 776. WHO, Geneva

    Google Scholar 

  5. 5.

    EPA, Arsenic, Inorganic (CASRN 7440-38-2) (1998)

  6. 6.

    Nakamura Y, Narukawa T, Yoshinaga J (2008) J Agric Food Chem 56:2536–2540

    Article  CAS  Google Scholar 

  7. 7.

    Almela C, Algora S, Benito V, Clemente MJ, Devesa V, Súñer MA, Vélez D, Montoro R (2002) J Agric Food Chem 50:918–923

    Article  CAS  Google Scholar 

  8. 8.

    Henrion A (1994) Fresenius J Anal Chem 350:657–658

    Article  CAS  Google Scholar 

  9. 9.

    Ellison SLR, Rosslein M, Williams A (2000) EURACHEM/CITAC guide: quantifying uncertainty in analytical measurement, 2nd edn. CITAC Secretariat, Teddington, pp 89–96

    Google Scholar 

  10. 10.

    Narukawa T, Inagaki K, Kuroiwa T, Chiba K (2008) Talanta 77:427–432

    Article  CAS  Google Scholar 

  11. 11.

    International Organization for Standardization (2003) ISO Guide 35, Reference Materials—general and statistical principles for certification. ISO, Geneva

    Google Scholar 

  12. 12.

    Linsinger TPJ, Pauwels J, Van der Veen AMH, Schimmel H, Lamberty A (2001) Accredit Qual Assur 6:20–25

    Article  CAS  Google Scholar 

  13. 13.

    International Organization for Standardization (2008) ISO/IEC Guide 98, Uncertainty of measurement—guide to the expression of uncertainty in measurement. ISO, Geneva

    Google Scholar 

  14. 14.

    Edmonds JS, Morita M, Shibata Y (1987) J Chem Soc, Perkin Trans 1:577–580

    Article  Google Scholar 

  15. 15.

    Raab A, Fecher P, Feldmann J (2005) Microchim Acta 151:153–166

    Article  CAS  Google Scholar 

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Correspondence to Koichi Chiba.

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Narukawa, T., Inagaki, K., Zhu, Y. et al. Preparation and certification of Hijiki reference material, NMIJ CRM 7405-a, from the edible marine algae hijiki (Hizikia fusiforme). Anal Bioanal Chem 402, 1713–1722 (2012).

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  • Certification
  • Certified reference material
  • Trace elements
  • Hijiki
  • Arsenic species
  • Speciation