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Dopamine-loaded chitosan nanoparticles: formulation and analytical characterization

Analytical and Bioanalytical Chemistry volume 400, pages 1997–2002 (2011)Cite this article

Abstract

The formulation and characterization of dopamine (DA)-loaded chitosan nanoparticles (CSNPs) are described as preliminary steps for the development of potential DA carrier systems intended for Parkinson’s disease treatment. For this purpose, CSNPs were firstly produced and, afterwards, they were incubated in a DA aqueous solution to promote neurotransmitter loading. The characterization of the resulting nanoparticles started with Fourier transform infrared spectroscopy analysis to ascertain the presence of DA in the nanocarrier, whereas X-ray photoelectron spectroscopy analysis provided evidence of the localization of DA on the nanoparticle surface. A quartz crystal microbalance with dissipation monitoring (QCM-D) was then exploited to investigate both swelling of CSNPs and interaction of DA with CSNPs. In particular, the QCM-D revealed that this interaction is fast and so this allows a stable nanostructured system to be obtained.

QCM-D investigation on dopamine/chitosan nanoparticles interaction

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Acknowledgements

This work was partly supported by grants to Dr. E. De Giglio (FAR 2009; University of Bari, Italy).

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Affiliations

  1. Department of Chemistry, Bari University, Aldo Moro, 70125, Bari, Italy

    Elvira De Giglio, Damiana Cafagna & Luigia Sabbatini

  2. Department of Pharmaceutical Chemistry, School of Pharmacy, Bari University, Aldo Moro, 70125, Bari, Italy

    Adriana Trapani

  3. Department of Chemistry and Industrial Chemistry, Pisa University, 56126, Pisa, Italy

    Stefania Cometa

Authors
  1. Elvira De Giglio
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  2. Adriana Trapani
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  3. Damiana Cafagna
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  4. Luigia Sabbatini
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  5. Stefania Cometa
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Correspondence to Elvira De Giglio.

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De Giglio, E., Trapani, A., Cafagna, D. et al. Dopamine-loaded chitosan nanoparticles: formulation and analytical characterization. Anal Bioanal Chem 400, 1997–2002 (2011). https://doi.org/10.1007/s00216-011-4962-y

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  • DOI: https://doi.org/10.1007/s00216-011-4962-y

Keywords

  • Chitosan
  • Nanoparticles
  • Dopamine
  • QCM-D
  • FT-IR
  • XPS