Synchrotron radiation phase-contrast X-ray CT imaging of acupuncture points


Three-dimensional (3D) topographic structures of acupuncture points were investigated by using synchrotron radiation in-line X-ray phase contrast computerized tomography. Two acupuncture points, named Zhongji (RN3) and Zusanli (ST36), were studied. We found an accumulation of microvessels at each acupuncture point region. Images of the tissues surrounding the acupuncture points do not show such kinds of structure. This is the first time that 3D images have revealed the specific structures of acupuncture points.

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  1. 1.

    Zhang Y, Yan XH, Liu CL et al (2006) Photoluminescence of acupuncture point “Waiqiu” in human superficial fascia. J Lumin 119–120:96–99

    Article  Google Scholar 

  2. 2.

    Yan XH, Zhang XY, Liu CL et al (2009) Do acupuncture points exist? Phys Med Biol 54(9):N143–N150

    Article  CAS  Google Scholar 

  3. 3.

    Andrew CA, Min P, Jessica RS et al (2010) Electrical impedance of acupuncture meridians: the relevance of subcutaneous collagenous bands. PLoS ONE 5(7):e11907

    Article  Google Scholar 

  4. 4.

    Langevin HM, Yandow JA (2002) Relationship of acupuncture points and meridians to connective tissue planes. Anat Rec 269(6):257–265

    Article  Google Scholar 

  5. 5.

    Fitzgerald R (2000) Phase-sensitive x-ray imaging. Phys Today 53:23–27

    Article  Google Scholar 

  6. 6.

    Momose A (2005) Recent advances in x-ray phase imaging. Jpn J Appl Phys 44:6355–6359

    Article  CAS  Google Scholar 

  7. 7.

    Mosose A (1995) Demonstration of phase-contrast X-ray computed tomography using an X-ray interferometer. Nucl Instrum Methods Phys Res A 352(3):622–628

    Article  Google Scholar 

  8. 8.

    Dilmanian FA, Zhong Z, Ren B et al (2000) Computed tomography of x-ray index of refraction using the diffraction enhanced imaging method. Phys Med Biol 45(4):933–946

    Article  CAS  Google Scholar 

  9. 9.

    Pfeiffer F, Kottler C, Bunk O, David C (2007) Hard X-ray phase tomography with low-brilliance sources. Phys Rev Lett 98:108105

    Article  CAS  Google Scholar 

  10. 10.

    Raven C, Snigirev A, Snigireva I et al (1996) Phase-contrast microtomography with coherent high-energy synchrotron x rays. Appl Phys Lett 69(13):1826–1828

    Article  CAS  Google Scholar 

  11. 11.

    Spanne P, Raven C, Snigireva I, Snigirev A (1999) In-line holography and phase-contrast microtomography with high energy x-rays. Phys Med Biol 44:741

    Article  CAS  Google Scholar 

  12. 12.

    Shen XY, Wang H (1999) Acupuncture and Moxibustion. People’s Medical Publishing House, Beijing, p 311 and 372

    Google Scholar 

  13. 13.

    Zheng LY, Xu ZX, Zhen XC et al (2003) Objective detection and labeling of the common acupuncture points in rabbits Shanghai. J Acupunct Moxibustion 22(5):26–29

    Google Scholar 

  14. 14.

    Chen RC, Xie HL, Luigi R et al (2010) Phase contrast micro-computed tomography of biological sample at SSRF. Tsinghua Sci Technol 15(1):102–107

    Article  Google Scholar 

  15. 15.

    Kak AC, Slaney M (1988) Principles of computerized tomographic imaging. IEEE, New York

  16. 16.

    Xie HL, Deng B, Du GH et al (2010) Advanced imaging technology and application of Shanghai synchrotron radiation source. Mod Phys 3:42–50

    Google Scholar 

  17. 17.

    Shanghai Synchrotron Radiation Facility (2011) SSRF homepage. Accessed 27 Feb 2011

  18. 18.

    Gao CJ, Chen EY, Cai GJ (2005) X-anatomy study and computer image analysis of distribution of microvessel density in pulmonary carcinoma. Anat Clin 10(2):98–100

    Google Scholar 

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This work was supported by the National Basic Research Program of China (no. 2006CB504509) and the Project of the State Key Program of the National Science Foundation of China (Grant no. 10635060). We also would like to thank the staff of SSRF BL13W for technical support and their help in treatment of IL-XPCT images.

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Correspondence to Xinyi Zhang.

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Published in the special issue Imaging Techniques with Synchrotron Radiation with Guest Editor Cyril Petibois.

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Zhang, D., Yan, X., Zhang, X. et al. Synchrotron radiation phase-contrast X-ray CT imaging of acupuncture points. Anal Bioanal Chem 401, 803–808 (2011).

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  • Acupuncture point
  • Synchrotron radiation
  • X-ray
  • Phase contrast
  • Microvessel