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Quantum dot-based array for sensitive detection of Escherichia coli

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Abstract

A fluorescent quantum dot-based antibody array, used in sandwich format, has been developed to detect Escherichia coli O157:H7. Numerous parameters such as solid support, optimal concentration of immunoreagents, blocking reagents, and assay time were optimized for array construction. Quantum dot-conjugated anti-IgG was used as the detecting system. The array allows the detection of E. coli O157:H7 at concentrations below 10 CFU mL−1 without sample enrichment, exhibiting an increase of three orders of magnitude in the limit of detection compared to ELISA. The interference caused by Gram (+) and Gram (−) bacteria was negligible at low concentrations of bacteria.

 

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Acknowledgments

CIBER-BBN is an initiative funded by the VI National R&D&i Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund.

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Authors and Affiliations

  1. Applied Molecular Receptors Group (AMRg), Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jorge Girona, 18-26, 08034, Barcelona, Spain

    Nuria Sanvicens, Núria Pascual, Javier Adrián, Francisco Sánchez-Baeza & M.-Pilar Marco

  2. Applied Molecular Receptors Group (AMRg), CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jorge Girona, 18-26, 08034, Barcelona, Spain

    Nuria Sanvicens, Núria Pascual, Javier Adrián, Francisco Sánchez-Baeza & M.-Pilar Marco

  3. Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain

    María Teresa Fernández-Argüelles, José Manuel Costa-Fernández & Alfredo Sanz-Medel

  4. Applied Molecular Receptors Group (AMRg), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jorge Girona, 18-26, 08034, Barcelona, Spain

    Núria Pascual

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  1. Nuria Sanvicens
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  2. Núria Pascual
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  3. María Teresa Fernández-Argüelles
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Correspondence to Núria Pascual.

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Sanvicens, N., Pascual, N., Fernández-Argüelles, M.T. et al. Quantum dot-based array for sensitive detection of Escherichia coli . Anal Bioanal Chem 399, 2755–2762 (2011). https://doi.org/10.1007/s00216-010-4624-5

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  • DOI: https://doi.org/10.1007/s00216-010-4624-5

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