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New extraction sorbent based on aptamers for the determination of ochratoxin A in red wine

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Abstract

A new solid phase extraction method based on aptamers, an oligosorbent, was developed and applied to the determination of ochratoxin A (OTA) from red wine. Two solid supports were chosen to immobilize OTA aptamer by covalent binding (cyanogen bromide-activated sepharose) or noncovalent binding (streptavidin-activated agarose). The resulting oligosorbents were evaluated in terms of retention, selectivity, and capacity. To assess the selectivity of the resulting oligosorbents, control supports made only of a solid support without immobilized aptamers were simultaneously studied. After optimization of the selective extraction procedure, extraction recoveries close to 100% were obtained on both materials. No retention was observed on the control supports. A similar capacity was also found for both oligosorbents. However, the immobilization by covalent bonding appeared more robust for the determination of OTA in the wine. A conventional sorbent and an immunoaffinity column were also applied to the determination of OTA in red wine to compare the potential of the various approaches for the treatment of such complex samples.

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Acknowledgment

This work was supported by the French National Research Agency (Mycodiag Project, ANR CES).

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Authors and Affiliations

  1. Department of Analytical and Bioanalytical Sciences and Miniaturization, ESPCI ParisTech, UMR PECSA 7195 (CNRS–UPMC–ESPCI ParisTech), 10 rue Vauquelin, 75231, Paris Cedex 05, France

    Florence Chapuis-Hugon, Aude du Boisbaudry, Benjamin Madru & Valérie Pichon

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  1. Florence Chapuis-Hugon
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  2. Aude du Boisbaudry
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  3. Benjamin Madru
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  4. Valérie Pichon
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Correspondence to Florence Chapuis-Hugon.

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Published in the special issue Advances in Analytical Separations with Guest Editors Yolanda Pico and Joan O. Grimalt.

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Chapuis-Hugon, F., du Boisbaudry, A., Madru, B. et al. New extraction sorbent based on aptamers for the determination of ochratoxin A in red wine. Anal Bioanal Chem 400, 1199–1207 (2011). https://doi.org/10.1007/s00216-010-4574-y

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  • DOI: https://doi.org/10.1007/s00216-010-4574-y

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