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Capillary electrophoresis with electrochemiluminescence detection: fundamental theory, apparatus, and applications

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Abstract

This review presents a comprehensive survey of recent progress on electrochemiluminescence (ECL) detection coupled with capillary electrophoresis (CE). The fundamental theories involved in CE-ECL, e.g., the mechanism involving both coreactant-based and inhibitor-based ECL, as well as the possible analytes to be detected by CE-ECL are summarized. Different schemes for the construction of CE-ECL apparatus, including methods for preparing the working electrode, approaches for addition of ECL reagents, ways to fabricate electrical decouplers, and factors affecting ECL efficiency are reviewed. Discussion of the literature related to the application of CE-ECL from January 2005 to September 2010 is sorted by the corresponding analyte matrixes, namely, the standard solution, urine, serum and plasma, and other matrixes. Finally, possible trends for CE-ECL in the near future are discussed.

CE-ECL for the detection of Ru(bpy)3 2+ labeled analytes Recent development showed that the coupling of electrochemiluminescence (ECL) to capillary electrophoresis (CE) can be used for the detection of a large number of analytes such as ECL coreactants, ECL inhibitors and Ru(bpy)3 2+ labeled analytes. This figure shows a typical example of using CE-ECL for the detection of protein labeled with Ru(bpy)3 2+.

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Acknowledgements

This project was financially supported by the NSFC (20735002, 20877019) and a special project of the Ministry of Health of the People’s Republic of China (200902009).

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Analysis and Detection, Fujian Provincial Key Laboratory of Analysis and Detection, Department of Chemistry, Technology for Food Safety, Fuzhou University, Fuzhou, 350002, China

    Longhua Guo, FengFu Fu & Guonan Chen

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  1. Longhua Guo
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  2. FengFu Fu
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Correspondence to Guonan Chen.

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Published in the special issue Analytical and Bioanalytical Science in China with guest editors Lihua Zhang, Qiankun Zhuang and Yukui Zhang.

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Guo, L., Fu, F. & Chen, G. Capillary electrophoresis with electrochemiluminescence detection: fundamental theory, apparatus, and applications. Anal Bioanal Chem 399, 3323–3343 (2011). https://doi.org/10.1007/s00216-010-4445-6

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