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Comparison and characterization of polysaccharides from natural and cultured Cordyceps using saccharide mapping

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Abstract

Comparison and characterization of polysaccharides from natural and cultured Cordyceps on the basis of their chemical characteristics such as glycosidic linkages were performed for the first time using saccharide mapping. The results showed that polysaccharides from most of the natural and cultured Cordyceps had similar responses to enzymatic digestion. These polysaccharides mainly contained (1→4)-β-D-glucosidic linkages, and (1→4)-α-glucosidic, (1→6)-α-glucosidic, 1,4-β-D-mannosidic, as well as (1→4)-α-D-galactosiduronic linkages also existed in some polysaccharides. Especially, natural and cultured Cordyceps polysaccharides could be discriminated on the basis of high performance liquid chromatography profiles of pectinase hydrolysates, which is helpful to control the quality of polysaccharides from Cordyceps.

The procedure and typical profile of saccharide mapping.

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Abbreviations

DAD:

diode-array detection

ELSD:

evaporative light scattering detector

ESI:

electrospray ionization

FrA:

fraction A

FrB:

fraction B

HPLC:

high-performance liquid chromatography

HPSEC:

high-performance size-exclusion chromatography

LC:

liquid chromatography

MS:

mass spectrometry

PMP:

1-phenyl-3-methyl-5-pyrazolone

WSPC:

water-soluble polysaccharides from Cordyceps

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Acknowledgements

This research was supported by grants from the National Natural Science Foundation of China (no. 30928033), the Science and Technology Development Fund of Macao (028/2006/A2), and the University of Macau (UL015A/09-Y2) to S.P.Li.

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Authors and Affiliations

  1. Institute of Chinese Medical Sciences, University of Macau, Macao, SAR, China

    Jia Guan, Jing Zhao, Kun Feng, De-Jun Hu & Shao-Ping Li

Authors
  1. Jia Guan
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  2. Jing Zhao
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  3. Kun Feng
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  4. De-Jun Hu
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  5. Shao-Ping Li
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Corresponding author

Correspondence to Shao-Ping Li.

Additional information

Jia Guan, Jing Zhao and Kun Feng contributed equally to this work.

Published in the special issue Analytical and Bioanalytical Science in China with Guest Editors Lihua Zhang, Qiankun Zhuang and Yukui Zhang.

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Guan, J., Zhao, J., Feng, K. et al. Comparison and characterization of polysaccharides from natural and cultured Cordyceps using saccharide mapping. Anal Bioanal Chem 399, 3465–3474 (2011). https://doi.org/10.1007/s00216-010-4396-y

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  • DOI: https://doi.org/10.1007/s00216-010-4396-y

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