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Dispersive liquid–liquid microextraction combined with high-performance liquid chromatography–tandem mass spectrometry for the identification and the accurate quantification by isotope dilution assay of Ochratoxin A in wine samples

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Abstract

A novel approach for the rapid analysis of ochratoxin A (OTA) in wine samples is presented. Mycotoxin was extracted and concentrated from matrix using dispersive liquid–liquid microextraction (DLLME). The final extract is analyzed by liquid chromatography coupled to positive electrospray ionization tandem mass spectrometry employing [2H5]-ochratoxin A as internal standard. Some important parameters, such as the nature and volume of extraction solvent and dispersive solvent, and salt effect were investigated and optimized to achieve the best extraction efficiency and higher enrichment factor. Under the optimum extraction condition, the method provided enrichment factor around 80 times and showed a high sensitivity with method detection and quantification limits of 0.005 and 0.015 ng mL−1, respectively. To test the accuracy of the analytical procedure, the optimized method was applied to the analysis of reference material T1755 (naturally contaminated white wine), with excellent results (accuracy of 103%) and showing a good precision with a CV (n = 6) of 5.8%. The proposed method, which is demonstrated to be quick, cheap, accurate and highly selective, was successfully applied to the analysis of Italian wines.

Analysis of ochratoxin A in wine by DLLME coupled to LC-MS/MS

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References

  1. Pfohl-Leszkowicz A, Manderville R (2007) Mol Nutr Food Res 51:61–99

    Article  Google Scholar 

  2. O’Brien E, Dietrich D (2005) CRC Crit Rev Toxicol 35:33–60

    Article  CAS  Google Scholar 

  3. Evaluation of certain Mycotoxins: Ochratoxin A, WHO food additives series 47/FAO food and nutrition paper 74, 2001

  4. Petkova-Botcharova T, Chernozemsky IN, Castegnaro M (1988) Food Addit Contam 5:299–301

    Article  Google Scholar 

  5. IARC Monographs on the evaluation of carcinogenic risks to humans, vol 56: 489, IARC, Geneva, Switzerland, 1993

  6. EFSA 2006. European food safety authority. Opinion of the scientific panel on contaminants in the food chain of the EFSA on a request from the commission related to ochratoxin A in food. (2006)EFSA J 365: 1

  7. Commission Regulation (EC) 1881/2006 (2006) Off J Eur Union L 364:5–24

    Google Scholar 

  8. Mateo R, Medina Á, Mateo EM, Mateo F, Jiménez M (2007) Int J Food Microbiol 119:79–83

    Article  CAS  Google Scholar 

  9. Visconti A, Perrone G, Cozzi G, Solfrizzo M (2008) Food Addit Contam 25:193–202

    Article  CAS  Google Scholar 

  10. Monaci L, Palmisano F (2004) Anal Bioanal Chem 378:96–103

    Article  CAS  Google Scholar 

  11. Barna Vetro I, Solti L, Teren L, Gyongyosi A, Szabo E, Wolfling A (1996) J Agric Food Chem 44:4071–4074

    Article  CAS  Google Scholar 

  12. Flajs D, Domijan AM, Ivic D, Cvjetkovic B, Peraica M (2009) Food Control 20:590–592

    Article  CAS  Google Scholar 

  13. Valenta H (1998) J Chromatogr A 815:75–92

    Article  CAS  Google Scholar 

  14. Visconti A, Pascale M, Centonze G (2001) J AOAC Int 84:1818–1827

    CAS  Google Scholar 

  15. Flamini R, Panighel A (2006) Mass Spectrom Rev 25:741–774

    Article  CAS  Google Scholar 

  16. Leitner A, Zöllner P, Paolillo A, Stroka J, Papadopoulou-Bouraoui A, Jaborek S, Anklam E, Lindner W (2002) Anal Chim Acta 453:33–41

    Article  CAS  Google Scholar 

  17. Bacaloni A, Cavaliere C, Faberi A, Pastorini E, Samperi R, Laganà A (2005) J Agric Food Chem 53:5518–5525

    Article  CAS  Google Scholar 

  18. Rychlik M, Asam S (2008) Anal Bioanal Chem 390:617–628

    Article  CAS  Google Scholar 

  19. Stokvis E, Rosing H, Beijnen JH (2005) Rapid Commun Mass Spectrom 19:401–407

    Article  CAS  Google Scholar 

  20. Rezaee M, Assadi Y, Hosseini MRM, Aghaee E, Ahmadi F, Berijani S (2006) J Chromatogr A 1116:1–9

    Article  CAS  Google Scholar 

  21. Nagaraju D, Huang S-D (2007) J Chromatogr A 1161:89–97

    Article  CAS  Google Scholar 

  22. García-López M, Rodríguez I, Cela R (2007) J Chromatogr A 1166:9–15

    Article  CAS  Google Scholar 

  23. Rezaee M, Yamini Y, Shariati S, Esrafili A, Shamsipur M (2009) J Chromatogr A 1216:1511–1514

    Article  CAS  Google Scholar 

  24. Fattahi N, Samadi S, Assadi Y, Hosseini MRM (2007) J Chromatogr A 1169:63–69

    Article  CAS  Google Scholar 

  25. Montes R, Rodríguez I, Ramil M, Rubí E, Cela R (2009) J Chromatogr A 1216:5459–5466

    Article  CAS  Google Scholar 

  26. Zhao E, Zhao W, Han L, Jiang S, Zhou Z (2007) J Chromatogr A 1175:137–140

    Article  CAS  Google Scholar 

  27. Commission Decision 2002/657/EC (2002) Off J Eur Communities L 221:8–36

    Google Scholar 

  28. Commission Regulation (EC) 401/2006 (2006) Off J Eur Union L 70:12–34

    Google Scholar 

Download references

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Authors and Affiliations

  1. Dipartimento di Scienze Farmaceutiche (DIFARMA), Università degli Studi di Salerno, via Ponte Don Melillo, 84084, Fisciano, SA, Italy

    Luca Campone, Anna Lisa Piccinelli & Luca Rastrelli

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  1. Luca Campone
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  2. Anna Lisa Piccinelli
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  3. Luca Rastrelli
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Correspondence to Anna Lisa Piccinelli.

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Campone, L., Piccinelli, A.L. & Rastrelli, L. Dispersive liquid–liquid microextraction combined with high-performance liquid chromatography–tandem mass spectrometry for the identification and the accurate quantification by isotope dilution assay of Ochratoxin A in wine samples. Anal Bioanal Chem 399, 1279–1286 (2011). https://doi.org/10.1007/s00216-010-4347-7

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  • DOI: https://doi.org/10.1007/s00216-010-4347-7

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