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A method for analysis of dimethyl selenide and dimethyl diselenide by LC-ICP-DRC-MS

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Abstract

The aim of this work was to develop a simple and fast high performance liquid chromatography–inductively coupled argon plasma (ICP) mass spectrometry (MS) method capable of separating and detecting the two volatile selenium species dimethyl selenide (DMeSe) and dimethyl diselenide (DMeDSe) in biological samples. Dimethyl selenide and dimethyl diselenide were separated on a short reversed phase column using an eluent containing 40% methanol and detected by dynamic reaction cell ICP-MS monitoring the 80Se isotope. The limit of detection was 8 nM for both species (corresponding to 0.6 and 1.3 μg Se/L for DMeDSe and DMeSe, respectively). Both compounds exhibited a linear signal–concentration relationship in the investigated concentration range of 0.1–1 μM with a precision on the determinations better than 3%. The method was applied for analysis of samples from cancer cell lines incubated with methylseleninic acid, selenomethionine, Se-methylselenocysteine, and sodium selenite. DMeDSe were detected in some samples. The method offers a simple and fast analysis of DMeDSe and DMeSe using standard liquid chromatography coupled with ICP-MS equipment and interfacing.

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Authors and Affiliations

  1. Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark

    Kristoffer Lunøe, Charlotte Gabel-Jensen, Stefan Stürup & Bente Gammelgaard

  2. Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 7, 1871, Frederiksberg, Denmark

    Søren Skov

Authors
  1. Kristoffer Lunøe
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  2. Søren Skov
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  3. Charlotte Gabel-Jensen
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  4. Stefan Stürup
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  5. Bente Gammelgaard
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Correspondence to Kristoffer Lunøe.

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Lunøe, K., Skov, S., Gabel-Jensen, C. et al. A method for analysis of dimethyl selenide and dimethyl diselenide by LC-ICP-DRC-MS. Anal Bioanal Chem 398, 3081–3086 (2010). https://doi.org/10.1007/s00216-010-4242-2

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  • DOI: https://doi.org/10.1007/s00216-010-4242-2

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