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Selective peracetic acid determination in the presence of hydrogen peroxide using a label free enzymatic method based on catalase

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Abstract

Peracetic acid (PAA) is selectively determined in the presence of hydrogen peroxide (H2O2) by using the self-indicating UV–Vis molecular absorption properties of catalase. The PAA reacts with the protein giving an intermediate (Cat-I) which is reduced back by the aminoacid core surrounding the hemegroup. Since the original form of the enzyme and the Cat-I have different UV–Vis absorption properties, the absorbance changes can be used for PAA determination. The H2O2/catalase reaction is extremely fast so that neither Cat-I compound nor kinetic interferences are observed. The method permits PAA determination in the 5 × 10−7 to 1.5 × 10−5 M range, the reproducibility being between 1% and 10%. Using this method, PAA has been successfully determined in water samples treated with commercial PAA/H2O2 biocides. A theoretical study has also been carried out for obtaining a mathematical model able to analytically describe the process.

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Acknowledgments

This work was supported by the Ministry of Education and Science (MEC) of Spain within the project CTQ2008-06751-C02-01/BQU, and by the LaCaixa-DGA 2008 project which is gratefully acknowledged.

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Authors and Affiliations

  1. Analytical Chemistry Department, Analytical Biosensor Group (GBA), Faculty of Sciences, Aragón Institute of Nanoscience (INA), University of Zaragoza, 50006, Zaragoza, Spain

    Javier Galbán, Vanesa Sanz & Susana de Marcos

Authors
  1. Javier Galbán
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  2. Vanesa Sanz
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  3. Susana de Marcos
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Correspondence to Javier Galbán.

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Galbán, J., Sanz, V. & de Marcos, S. Selective peracetic acid determination in the presence of hydrogen peroxide using a label free enzymatic method based on catalase. Anal Bioanal Chem 398, 2117–2124 (2010). https://doi.org/10.1007/s00216-010-4145-2

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  • DOI: https://doi.org/10.1007/s00216-010-4145-2

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