Abstract
Kalanchoe pinnata (Lam.) Pers. (syn. Bryophyllum pinnatum; family Crassulaceae) is a popular plant used in traditional medicine in many temperate regions of the world and particularly in South America. In Guyana, the leaves are traditionally used as an anti-inflammatory and antiseptic to treat coughs, ulcers, and sores. The purpose of this study was to implement a method for targeting and identifying molecules with antimicrobial activity, which could replace chemical preservatives in cosmetic applications. The leaves were extracted by a method based on pressurized liquid extraction (PLE), using different solvents. A study of antimicrobial activity and cytotoxicity tests were performed to select the most interesting extract. To isolate one or more active molecules, the selected crude extract was fractionated by centrifugal partition chromatography (CPC) and then antimicrobial activity and cytotoxicity of each fraction were tested under the same procedure. The last step consisted of identifying the main compounds in the most active fraction by LC-MS/MS.
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Acknowledgement
This project was supported by the French competitiveness cluster “Cosmétique, Sciences de la Beauté et du Bien être”. The authors thank the Conseil Régional du Centre (France), the Conseil Général du Loiret (France), the Communauté d’agglomération d’Orléans and the Ville d’Orléans for their financial support. The authors acknowledge Jean-Marc Seigneuret and Emilie Dufour (Adonis, Alban Muller, France) for their help and providing the Kalanchoe pinnata leaves and, Marie-Pierre Papet for her advice.
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El Abdellaoui, S., Destandau, E., Toribio, A. et al. Bioactive molecules in Kalanchoe pinnata leaves: extraction, purification, and identification. Anal Bioanal Chem 398, 1329–1338 (2010). https://doi.org/10.1007/s00216-010-4047-3
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DOI: https://doi.org/10.1007/s00216-010-4047-3