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Evaluation of nucleic acid duplex formation on gold over layers in biosensor fabricated using Czochralski-grown single-crystal silicon substrate

Analytical and Bioanalytical Chemistry volume 398, pages 751–758 (2010)Cite this article

Abstract

With a view to developing an economical and elegant biosensor chip, we compared the efficiencies of biosensors that use gold-coated single-crystal silicon and amorphous glass substrates. The reflectivity of light over a wide range of wavelengths was higher from gold layer coated single-crystal silicon substrates than from glass substrates. Furthermore, the efficiency of reflection from gold layers of two different thicknesses was examined. The thicker gold layer (100 nm) on the single-crystal silicon showed a higher reflectivity than the thinner gold film (10 nm). The formation of a nucleic acid duplex and aptamer–ligand interactions were evaluated on these gold layers, and a crystalline silicon substrate coated with the 100-nm-thick gold layer is proposed as an alternative substrate for studies of interactions of biomolecules.

Reflection based biosensor. Thin gold-layer coated on Czochralski-grown single-crystal silicon. Thiolated DNA hybridized with RNA-aptamer followed by protein was attached to obtain the reflection changes.

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Affiliations

  1. Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8562, Japan

    Subash C. B. Gopinath, Koichi Awazu & Makoto Fujimaki

  2. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, 657-8501, Japan

    Ramanujam Kumaresan & Minoru Mizuhata

  3. Nanodevice Innovation Research Center, AIST, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8562, Japan

    Subash C. B. Gopinath & Junji Tominaga

  4. Biomedical Research Institute, AIST, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan

    Penmetcha K. R. Kumar

Authors
  1. Subash C. B. Gopinath
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  2. Ramanujam Kumaresan
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  3. Koichi Awazu
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  4. Makoto Fujimaki
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  5. Minoru Mizuhata
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  6. Junji Tominaga
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  7. Penmetcha K. R. Kumar
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Correspondence to Subash C. B. Gopinath.

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Gopinath, S.C.B., Kumaresan, R., Awazu, K. et al. Evaluation of nucleic acid duplex formation on gold over layers in biosensor fabricated using Czochralski-grown single-crystal silicon substrate. Anal Bioanal Chem 398, 751–758 (2010). https://doi.org/10.1007/s00216-010-3994-z

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  • DOI: https://doi.org/10.1007/s00216-010-3994-z

Keywords

  • Spectrophotometry
  • Crystal
  • Amorphous
  • Au layer
  • DNA hybridization