Comparison of CIM discs and CPG glass as solid supports for bioanalytical columns used in allergen detection
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This work presents a comparison of convective interaction media (CIM) and controlled pore glass (CPG) as solid supports for immunoglobulin antibodies used in bioanalytical detection of allergens in foodstuffs. A flow-injection manifold with highly sensitive thermal lens spectrometric detection was used for this purpose. Using beta-lactoglobulin, a milk allergen, as a model analyte, CIM disc supports had a higher linear range (0.2–3.5 μg L−1), better reproducibility (intra-day RSD = 1%, inter-day RSD = 10%), lower consumption of reagents, and better immunocolumn stability (1 month, over 240 injections of substrate), while providing comparable LODs (0.1 μg L−1). Application of CIM discs as solid supports in immunocolumns for allergen detection enables fast and sensitive screening of allergens in foodstuffs with sample throughput of up to eight samples per hour.
KeywordsBioanalytical columns Controlled-pore glass (CPG) Convective interaction media (CIM) Allergen detection Thermal lens spectrometry
Convective interaction media
Controlled pore glass
Thermal lens spectrometry
Flow injection analysis
Enzyme linked immunosorbent assay
This work was financially supported by the Slovenian Research Agency, through research program P1-0034 and a young researcher’s grant to Ana Čevdek. Financial support for equipment was obtained from the EU and the Municipality of Nova Gorica through structural funds contract C1536-08S330265. The authors are also grateful to BIA Separations, for their kind donation of diol CIM micro discs, and to Dr Aleš Podgornik for inspiring discussions.
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