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Analytical and Bioanalytical Chemistry

, Volume 398, Issue 1, pp 555–562 | Cite as

Comparison of CIM discs and CPG glass as solid supports for bioanalytical columns used in allergen detection

  • Ana Čevdek
  • Mladen FrankoEmail author
Original Paper

Abstract

This work presents a comparison of convective interaction media (CIM) and controlled pore glass (CPG) as solid supports for immunoglobulin antibodies used in bioanalytical detection of allergens in foodstuffs. A flow-injection manifold with highly sensitive thermal lens spectrometric detection was used for this purpose. Using beta-lactoglobulin, a milk allergen, as a model analyte, CIM disc supports had a higher linear range (0.2–3.5 μg L−1), better reproducibility (intra-day RSD = 1%, inter-day RSD = 10%), lower consumption of reagents, and better immunocolumn stability (1 month, over 240 injections of substrate), while providing comparable LODs (0.1 μg L−1). Application of CIM discs as solid supports in immunocolumns for allergen detection enables fast and sensitive screening of allergens in foodstuffs with sample throughput of up to eight samples per hour.

Keywords

Bioanalytical columns Controlled-pore glass (CPG) Convective interaction media (CIM) Allergen detection Thermal lens spectrometry 

Abbreviations

BLG

β-Lactoglobulin

CIM

Convective interaction media

CPG

Controlled pore glass

TLS

Thermal lens spectrometry

FIA

Flow injection analysis

ELISA

Enzyme linked immunosorbent assay

Notes

Acknowledgment

This work was financially supported by the Slovenian Research Agency, through research program P1-0034 and a young researcher’s grant to Ana Čevdek. Financial support for equipment was obtained from the EU and the Municipality of Nova Gorica through structural funds contract C1536-08S330265. The authors are also grateful to BIA Separations, for their kind donation of diol CIM micro discs, and to Dr Aleš Podgornik for inspiring discussions.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Laboratory for Environmental ResearchUniversity of Nova GoricaNova GoricaSlovenia

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